Axiom Biotechnologies, Inc.

Vivia Biotech S.l.

Saryna Biotechnologies LLC

Alex Okun

Ilya Okun

John Ransom

Teresa A Bennett

Gregory Kaler

Andrew Beernink

David J Sieg

Disclosed herein is a sample supply device that alternates between the supply of samples from one sample line while cleaning a second sample line and then supplying a second sample from the second sample line while cleaning the first sample line. This is repeated in rapid succession to allow greater speed in analyzing a plurality of samples in a shorter amount of time.

Methods for multiplexed sample analysis

A system and method of mixing and injecting discrete sample mixtures into a flow cytometer or other sample analysis apparatus may generally comprise a sample injection guide coupling a liquid handling apparatus with a sample analysis apparatus and facilitating injection of discrete sample mixtures into a fluidic system of the apparatus.

Direct mixing and injection for high throughput fluidic systems

High speed sample supply device

An apparatus and method for real-time measurement of a cellular response of a test compound or series of test compounds ( ) on a flowing suspension of cells ( ), in which a homogenous suspension of each member of a series of cell types ( ) is combined with a concentration of a test compound ( ), directed through a detection zone ( ), and a cellular response of the living cells is measured in real time as the cells in the test mixture are flowing through the detection zone ( ). The apparatus may be used in automated screening of libraries of compounds, and is capable of real-time variation of concentrations of test and standard compounds and generation of dose/response profiles within a short timespan.

Apparatus and method for real-time measurement of cellular response

Sensitive methods for identifying compounds having biological activity comprising combining living cells with two fluorescent membrane permeable ionic dyes having the same charge sign, the first of which has an emission spectrum which overlaps the excitation spectrum of the second fluorescent membrane penetrative dye. The fluorescence is then induced by illuminating the dyes at a wavelength corresponding to the excitation spectrum of the first fluorescent dye and emission is then registered at a wavelength corresponding to the emission spectrum of the second fluorescent dye (FRET). The change in the FRET is indicative of a modulation of cell membrane potential by the biologically active compounds.

Methods of registering trans-membrane electric potentials

An apparatus and method for real-time measurement of a cellular response of a test compound or series of test compounds (,) on a flowing suspension of cells (,), in which a homogeneous suspension of each member of a series of cell types (,) is combined with a concentration of a test compound (,), directed through a detection zone (,), and a cellular response of the living cells is measured in real time as the cells in the test mixture are flowing through the detection zone (,). The apparatus may be used in automated screening of libraries of compounds, and is capable of real-time variation of concentrations of test and standard compounds and generation of dose/response profiles within a short time span.

Cell flow apparatus and method for real-time of cellular responses

Cell flow apparatus and method for real-time measurements of cellular responses

An apparatus and method for real-time measurement of a cellular response of a test compound or series of test compounds (303) on a flowing suspension of cells (349), in which a homogeneous suspension of each member of a series of cell types (349) is combined with a concentration of a test compound (303), directed through a detection zone (355), and a cellular response of the living cells is measured in real time as the cells in the test mixture are flowing through the detection zone (355). The apparatus may be used in automated screening of libraries of compounds, and is capable of real-time variation of concentrations of test and standard compounds and generation of dose/response profiles within a short timespan.

Apparatus and method for compound profiling of living cells

An apparatus and method for real-time measurement of a cellular response of a test compound or series of test compounds on a flowing suspension of cells in which a homogeneous suspension of living cells is combined with a concentration of a test compound, directed through a detection zone, and a cellular response of the living cells is measured in real time as the cells in the test mixture are flowing through the detection zone. The apparatus may be used in automated screening of libraries of compounds, and is capable of real-time variation of concentrations of test and standard compounds and generation of dose/response profiles within a short timespan.

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San Diego, CA, United States of America

Alex Okun