General Electric Company

Global Life Sciences Solutions USA LLC

Leica Microsystems Cms Gmbh

Alex David Corwin

Kashan Ali Shaikh

Weston Blaine Griffin

Reginald Donovan Smith

Brian Michael Davis

Xiaohua Zhang

Chengkun Zhang

Vandana Keskar

Zhen Liu

David Andrew Shoudy

Kevin Bernard Kenny

Christine Lynne Surrette

Robert John Filkins

Christine Lynne Pitner

Jun Xie

Stephen Eric Zingelewicz

John Richard Nelson

Kanakasabapathi Subramanian

Christopher Michael Puleo

Erik Leeming Kvam

Ralf Lenigk

Todd Frederick Miller

Kuna Venkat Satya Rama Kishore

Wei Gao

Brian Joseph Scherer

Nannan Chen

Jessica Godin Karp

Pei-Hsin Kuo

Michael S Lazare

Meret Vollenweider

Christopher Fred Keimel

Marco Francesco Aimi

Reed Roeder Corderman

Michael John Gerdes

Xuefeng Wang

Atanu Saha

Fiona Ginty

Parag Thakre

Bo Li

Christopher James Sevinsky

Xiaofeng Liu

David LaVan Henderson

Shubhra Bansal

Antti Seppo

Jessica Marie Godin

Eddula Sudhakar Reddy

Adriana Ines Larriera

Thomas Ha

Natalia R Jun

Ainura Kyshtoobayeva

Denise A Hollman-Hewgley

Ying Li

Tyler John Hammond

Christine Lynne Surette

In an embodiment, a tubing module for a bioprocessing system includes a first tubing holder block configured to receive at least one pump tube and hold the at least one pump tube in position for selective engagement with a peristaltic pump, a second tubing holder block configured to receive a plurality of pinch valve tubes and hold each pinch valve tube of the plurality of pinch valve tubes in position for selective engagement with a respective actuator of a pinch valve array, and wherein the first tubing holder block and the second tubing holder block are interconnected.

Apparatus for fluid line management in a bioprocessing system

A bioprocessing system includes a first fluid assembly having a first fluid assembly line connected to a first port of a first bioreactor vessel though a first bioreactor line of a first bioreactor vessel, a second fluid assembly having a second fluid assembly line connected to a second port of the first bioreactor vessel through a second bioreactor line of the first bioreactor vessel, and an interconnect line providing for fluid communication between the first fluid assembly and the second fluid assembly, and for fluid communication between the second bioreactor line of the first bioreactor vessel and the first bioreactor line of the first bioreactor vessel.

System and method for fluid flow management in a bioprocessing system

An apparatus for bioprocessing includes a housing, a drawer receivable within the housing, the drawer including a plurality of sidewalls and a bottom defining a processing chamber, and a generally open top, the drawer being movable between a closed position in which the drawer is received within the housing, and an open position in which the drawer extends from the housing enabling access to the processing chamber through the open top, and at least one bed plate positioned within the processing chamber and configured to receive a bioreactor vessel.

Bioprocessing apparatus

A bioprocessing system includes a first module configured for enriching and isolating a population of cells, a second module configured for activating, genetically modifying, and expanding the population of cells, and a third module configured for harvesting the expanded population of cells.

Systems and methods for bioprocessing

A fluidic handling unit includes a baseplate, a fluidic inlet block, a fluidic outlet block, a pump in fluidic communication with the fluidic inlet block and the fluidic outlet block, a carrier control board in electrical communication with the pump, and a flow cell carrier comprising a microfluidic flow cell receiving area, wherein the flow cell carrier is configured to receive and retain the fluidic handling unit. A bottom surface of the fluidic handling unit is configured to complementary mate with a top surface of the flow cell carrier, or wherein a bottom surface of the flow cell carrier is configured to complementarily mate with a top surface of the fluidic handling unit.

Self-contained slide processing unit for biological specimens

The present disclosure relates to approaches for assessing a sample or the presence of microorganisms. The sample, in certain implementations may be assessed for one or both of absence of microorganisms (sterility) and/or for concentration of said organisms (bio-burden). sample partition device may be employed that partitions the sample input volume into multiple discrete measurement zones with little or no loss of sample (e.g., zero-loss) and with little operator involvement, thereby reducing operator- and environment-based false positives.

Assessment of micro-organism presence

The present disclosure relates to a consumable sample partition device and it assembly and use. The sample partition device can be used to test a sample for absence of microorganisms (sterility) and/or for concentration of said organisms (bio-burden). The sample partition device partitions the sample input volume into multiple discrete measurement zones with little or no loss of sample (e.g., zero-loss) and with little operator involvement, thereby reducing operator- and environment-based false positives.

A method for generating an image of a region of interest in a biological sample comprising the steps of: generating a first image including the region of interest of the biological sample having undergone a first protocol but not a second protocol; and generating a second image including the region of interest of the biological sample after having undergone a second protocol; wherein the region of interest is smaller than said sample. Also provided is a method of analyzing a biological sample, comprising providing an image of the biological sample according to the method for generating an image of a region of interest in a biological sample, and analyzing the biological sample from the image. Further provided are system and kit that comprise the means for executing the novel methods.

Methods for generating an image of a biological sample

Systems and methods for processing biological specimens are provided. The biological specimen processing system generally includes a flow cell carrier for holding a microfluidic flow cell and a fluidic handling unit attachable to the flow cell carrier. The fluidic handling unit interfaces with the microfluidic flow cell and can include fluidic pumps, fluidic connections, integrated electronics, and processing software to facilitate processing of a biological specimen contained in the microfluidic flow cell.

A bioreactor vessel includes a bottom plate, a vessel body coupled to the bottom plate, the vessel body and the bottom plate defining an interior compartment therebetween, and a plurality of recesses formed in the bottom plate, each recess of the plurality of recesses being configured to receive a corresponding alignment pin on a bed plate for aligning the bioreactor vessel on the bed plate.

Bioprocessing vessel

Approaches are disclosed for calibrating a plurality of imaging devices, such as microscopes. In certain implementations, a calibration plate is employed that includes a variety of calibration features. The calibration features comprise a geometric calibration, an illumination calibration, and an optical calibration. Imaging devices calibrated in accordance with the present approaches may be used to generate images having consistent attributes, such as brightness, regardless of which imaging device is employed.

Calibration of microscopy systems

A system for extracting material from a region of interest includes a fluid delivery base comprising an inlet channel and an outlet channel formed within the fluid delivery base, a gasket affixed to the fluid delivery base, wherein the gasket comprises at least one opening exposing an open end of the inlet channel and an open end of the outlet channel; a support comprising a sample-supporting surface facing the gasket and an opposing surface; and an alignment member coupled to the opposing surface in a fixed position and such that the support is positioned between the fluid delivery base and the alignment member, wherein one or both of the alignment member or the fluid delivery base are biased towards one another by a force (e.g., a magnet or spring force) and wherein the fluid delivery base is separable from the support and configured to move along a plane of the sample-supporting surface to align with the alignment member.

Extraction of materials from regions of interest in a sample

The present approach relates to the measure of the planar tilt of a slide on a microscope using an integrated auto-focuser. The tilt of the slide can be used to detect improperly loaded slides (i.e. if the slide is resting on either the cover-slip or a printed barcode), compensate for misalignment between the microscope optical axis and the slide, and reduce subsequent focusing times.

Determination of deflection of a microscope slide

Growing community of inventors

Niskayuna, NY, United States of America

Alex David Corwin