Canadian Patents & Development Ltd.

Aleksandra Dmochowska

Howard Bussey

David Y Thomas

This invention is concerned with the specific processing of secreted proteins in genetically modified yeast cells. The yeast KEX1 gene was cloned and the KEX1 product was shown to be a serine protease, evidently a carboxypeptidase B-like protease. A probable site of processing of polypeptides by the KEX1 gene product is at the C-terminus of the .alpha. subunit of the killer toxin, where the mature toxin subunit is followed in the precursor by a pair of basic amino acid residues. Processing likely involves an endoprotease cut following these basic residues, and their subsequent C-terminal trimming by a carboxypeptidase. Consistent with the KEX1 product being this carboxypeptidase is the finding that it is also involved in .alpha.-factor pheromone production. In wildtype yeast, KEX1 is not essential for .alpha.-factor production, as the final hormone repeat in the prepro .alpha.-hormone precursor does not need C-terminal processing to form one copy of the active hormone. However, in a mutant strain where .alpha.-factor production requires carboxypeptidase B-like processing, pheromone production is KEX1 dependent. Besides the processing of yeasts' naturally secreted proteins, of which .alpha.-factor pheromone and K1 killer toxin are the best characterized examples, the products of KEX1 and KEX2 are required for the processing of some proteins and peptides of commercial importance for example hormones and neuropeptides. High level production of certain commercially important proteins and peptides appears to require the overproduction of the appropriate processing proteases. The cloning of KEX1 on a multicopy plasmid can provide for this overproduction.

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Warsaw, Poland

Aleksandra Dmochowska