Fluicell Ab

Other

Cellectricon Ab

Nanoxis Consulting Ab

Aldo Jesorka

Owe Orwar

Alar Ainla

Mattias Karlsson

Johan Pihl

Kent Jardemark

Jessica Olofsson

Eskil Sahlin

Kiryl Kustanovich

Mehrnaz Shaali

Anders Karlsson

Gavin Jeffries

Ventislav Mitkov Yanchev

Max Davidson

Tatiana Lobovkina

Irep Gözen

Roger Karlsson

A surface acoustic wave resonant sensor for measuring a sample comprising a single port surface acoustic wave (SAW) resonator comprising an interdigital transducer and at least one reflective grating. The sensor is provided with a region for receiving the sample, said region being in communication with the at least one reflective grating and the IDT is separated acoustically and electrically from the region for receiving a sample such that the IDT is not mass sensitive to the sample. The sensor is especially suitable for bio sensing applications.

Surface acoustic wave resonant sensor

Here we disclose a lithographic pattern development process for amorphous fluoropolymers. Amorphous fluoropolymers are a class of plastic materials with high chemical inertness and favorable optical properties. Exposure of surface-deposited layers of such polymer with high energy radiation leads to a change in the chemical structure of the polymer, which selectively compromises the solubility of the exposed areas in fluorinated organic solvents. Micro- and nanopatterning with a feature size down to &#x3c;50 nm was achieved by dissolving and removing unexposed amorphous fluoropolymer from exposed, surface deposited films. The amorphous fluoropolymer functions thus as a negative resist.

Lithographic pattern development process for amorphous fluoropolymer

One embodiment of the invention provides a system adapted and configured to generate a localized flow circulation zone. The system includes: a free-standing microfluidic pipette comprising three or more channels with exits separated from each other by an outer surface of the pipette; and a controller programmed to control fluid flows through each of the three or more channels to generate a localized recirculating fluid flow path outside the pipette. Liquid leaving the microfluidic pipette through at least one outlet channel exit is withdrawn through at least two inlet channel exits.

Pipettes, methods of use, and methods of stimulating an object of interest

Aspects of the present invention provide a freestanding microfluidic pipette with integrated wells for solution storage. Further aspects of the invention provide a holding interface to provide connectivity with external control components. One aspect of the invention provides a system for applying a microfluidic device in microscopy. The system includes: a microfluidic device having an elongated shape and defining one or more wells for solution storage and processing; and an interface adapted and configured to hold the microfluidic device in a freestanding manner and facilitate simultaneous connection of the one or more wells with a flow controller. Another aspect of the invention provides a method for utilizing a microfluidic device. The method includes: providing a device as described herein; positioning the device adjacent to a microscope; and actuating the interface to operate the microfluidic device.

Microfluidic device with holding interface, and methods of use

The present invention features a freestanding microfluidic pipette with both solution exchange capability and fluid re-circulation, enabling highly localized and contamination- free fluid delivery within a confined volume in the vicinity of the pipette exit. Preferably, the device features direct positioning, so the pipette can be directed at a point or object of interest, such as a biological or artificial cell, a defined surface area or a sensor element within an open volume, using micro- or nanopositioning techniques. One aspect of the invention provides a free-standing pipette. The free-standing pipette includes a microfluidic device comprising one or more channels with exits leading into an open-volume and a positioning device programmed to hold the microfluidic device at an angle from a horizontal axis.

The present invention relates to methods of measuring electrical properties of a cell using electrode devices comprising tapered nanotips having submicrometer dimensions ('nanoelectrodes') for insertion into a cell. The devices are used to measure electrical properties of the cell and, optionally, may be used to electroporate, the cell or subcellular structures within the cell. The invention also provides arrays of electrode devices having nanotips for simultaneously or sequentially measuring the electrical properties of cells (e.g., such as surface immobilized cells). The electrodes can be used to measure properties of ion channels and in HTS assays to identify drugs which affect the properties of ion channels. The invention additionally provides microfluidic systems adapted for use with the electrode devices having nanotips. In combination with the electrodes, the microfluidic systems provide cell-based biosensors for monitoring cellular responses to conditions, such as exposure to candidate drugs.

Nanoelectrodes and nanotips for recording transmembrane currents in a plurality of cells

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Gothenburg, Sweden

Aldo Jesorka