Stemcell Technologies Inc.

Albertus Wernerus Wognum

Peter Michael Lansdorp

Ning Yuan

The present disclosure provides a method of determining the frequency and proliferative potential of progenitor cells in a starting cell preparation comprising (a) culturing the cell preparation for 5-10 days in a culture medium comprising FLT-3 Ligand (FL) and Thrombopoietin (TPO); (b) detecting and enumerating the colonies; and (c) assessing the frequency and proliferative potential of progenitor cells in the starting cell preparation based on the number of colonies in step (b). The number of colonies in step (b) correlates to the number of colonies in a standard 14-day colony-forming cell assay. The method is particularly useful for predicting and evaluating the suitability of grafts for transplantation.

Shorter colony-forming cell assay

A method for preventing or inhibiting non-specific binding reactions between a detection reagent and an antigen in an immunological assay is described. The method involves using tetrameric antibody complexes that can bind to the antigen and are comprised of two monoclonal antibodies of a first animal species linked to two monoclonal antibodies of a second animal species that can bind the antibodies of the first animal species. Preferably, the antigen is an Fc Receptor and the method reduces the binding of a detection antibody with Fc receptors present on the surface of many cells.

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Vancouver, Canada

Albertus Wernerus Wognum