Monsanto Technology LLC

Alberto Iandolino

Shihshieh Huang

Michael J Bennett

Gregory John Peel

Wei Zheng

Steven H Schwartz

Janette V Oakes

Bill L Hendrix

Sharon E Radke

Juan Pedro Sanchez

Heather A Lindfors

Yao Luo

Rick C Taytroe

Andrew Walter Mroczka

Semi-automated quantitative phenotype analysis of organisms, such as plant organisms, bacterial organisms, and the like. An imaging system, image capture specifications, a semi-automated quantitative image analysis process, and automated batch processing of acquired images that enables completely quantitative experimental readouts that produces data rapidly and objectively.

Systems and methods for quantitative phenotype analysis

Transfection of plant cells with dsRNA through foliar application encounters cuticle, cell wall and plasmalemma three major barriers. We developed cationic polymer and sugar based formulations and protocols that can effectively deliver dsRNA into plant cells resulted in gene silencing. This disclosure covers the novel methods to deliver dsRNA into plant suspension cells with 'one step' treatment and plant foliar cells with 'one step' topical application.

Methods and compositions for delivering nucleic acids to plant cells and regulating gene expression

The present disclosure provides compositions and methods for regulating gene expression via RNA-mediated silencing. The present disclosure also provides compositions and methods to optimize the processing of a dsRNA molecule into small RNA duplexes. The present disclosure further provides compositions and methods to improve the efficiency of a dsRNA molecule in producing desired small RNAs and promoting the silencing of a gene of interest.

Compositions and methods for regulating gene expression via RNA interference

The present disclosure provides compositions and methods for delivering a polynucleotide from the exterior surface of a plant or plant part into the interior of a plant cell. More specifically, the present disclosure relates to compositions comprising at least one polynucleotide and at least one agent that is able to disrupt at least one barrier of the plant or plant part.

Compositions and methods for delivery of a polynucleotide into a plant

The present disclosure provides compositions and methods for introducing nucleic acids into plants. More specifically, disclosed herein are methods and compositions for introducing a nucleic acid, such as a double-stranded RNA, for silencing a target gene in a plant. Also disclosed herein are plants exhibiting modification of a target gene, obtained through the use of such methods and compositions.

Methods and compositions for introducing nucleic acids into plants

Growing community of inventors

Davis, CA, United States of America

Alberto Iandolino