Life Technologies Corporation

Invitrogen Corporation

The United States of America As Represented by the Department of Health

The United States of America As Represented by the Secretary of

United States Government As Represented by the Secretary of the Dept. of

Alberto Haces

Valentina C Ciccarone

John S Driscoll

Mu-Ill Lim

Victor E Marquez

Robert I Glazer

Theodore Breitman

Christopher K-h Tseng

Christopher K Tseng

The present invention discloses highly packed polycationic ammonium, sulfonium and phosphonium lipid compounds useful for making lipid aggregates for delivery of macromolecules and other compounds into cells. They are especially useful for the DNA-dependent transformation of cells. Methods for their preparation and use as intracellular delivery agents are also disclosed.

Highly-packed polycationic ammonium, sulfonium and phosphonium lipids

Highly packed polycationic ammonium, sulfonium and phosphonium lipids

Novel assay methods employing compounds which are chemically or enzymatically cleavable and which give rise to an intermediate which further decomposes by an intramolecular anchimeric displacement reaction which releases a signal producing species are disclosed. Also disclosed are probe hybridization assays employing the compounds of the invention employing thermostable enzymes which are not denatured by the hybridization conditions. Such signal producing species may include chemiluminescent dioxetanes and other colored products.

Methods for generating light with chemiluminescent dioxetanes activated

Several analogues of hexamethylene bis[acetamide] were found to be effective differentiating agents. The most effective of these compounds was 3,3'-(1,6-hexandiyl)bis[5,5-dimethyl-2,4-imidazolinedione].

Chemical differentiating agents

Cyclopentenyl pyrimidine compounds have potent anti-viral, anti-tumor and differentiating activity. Of these compounds, cyclopentenyl cytosine has proved to be particularly effective in a variety of tumors, as well as having good antiviral activity and potent differentiating properties.

Antiviral and anticancer cyclopentenyl cytosine

The new compound 3-deazaneplanocin A has been discovered to have potent anti-viral, anti-tumor activity and differentiating activity. A simple method for preparing 3-deazaneplanocin A has been developed involving nucleophilic substitution, which method can also be used to prepare a great variety of carbocyclic nucleosides.

3-deazaneplanocin, intermediates for it, and antiviral composition and

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Gaithersburg, MD, United States of America

Alberto Haces