E.i. Dupont De Nemours and Company

Tate & Lyle Ingredients Americas LLC

A E Staley Manufacturing Company

Albert W Alsop

Robert Donald Fallon

Scott Christopher Jackson

Qiong Cheng

Pierre E Rouviere

Stephen R Fahnestock

David Neil Marks

F Glenn Gallagher

Tanja Maria Gruber

Mayis Seapan

William F Lehnhardt

Tyler T Ames

John M Iwasyk

Robert E Trotter

James M Gaddy

Linda Jane Solomon

Luis Alberto Chu

Gregory M Wenndt

Bryan C Dravis

Eugene K Yu

Michael L Luyben

Jefferson C Lievense

Patrick Fitzgibbon

Abigail K Luckring

Michael P Perry

Edwin R Hendrickson

Jan M Stouffer

Elwood Neal Blanchard

Sharon Jo Keeler

Jeffrey David Cohen

Clara Young Lin

David A Douglas, Jr

James S Disney

Dennis M Adkesson

Sheryl M Anyzek

Earl W Blackwell

Dennis N Adkesson

James M Disney

Dennis Michael Adkesson

Disclosed herein is introduction of different nutrient solution formulations to a subterranean target site to prevent biofilm formation and biomass aggregation at the sand face of an injection well bore during MEOR or bioremediation processes. A first nutrient solution formulation supports growth of introduced microorganisms and a second nutrient solution formulation is used to promote biofilm formation to allow plugging of pores and channels in the subterranean target site, for MEOR or bioremediation.

Prevention of biomass aggregation at injection wells

The invention relates to the recombinant expression of a peptide of interest in the form of a fusion protein comprising a solubility tag. The fusion protein comprises at least two portions separated by a cleavable peptide sequence wherein one portion is devoid of cysteine residues and the second portion comprises an effective number of cross-linkable cysteine residues. After cell lysis and isolation of the fusion protein, the fusion protein is subsequently cleaved into a mixture of first and second portions. Oxidative cross-linking is used to selectively precipitate one of the two portions to facilitate simple and effective separation of the peptide of interest.

Recombinant peptide production using a cross-linkable solubility tag

Methods are provided for treating production fluid in an oil reservoir to reduce the amount of sulfide in the production fluid. The production fluid is treated with nitrate and/or nitrite ions in an aqueous solution that is added to an injection well that is in contact with the production well.

Reducing sulfide in oil reservoir production fluids

A method to improve the effectiveness of MEOR or bioremediation processes. In this method toxic chemicals accumulated in subterranean sites adjacent to the water injection wells are either dispersed or removed prior to introduction of microbial inocula for enhanced microbial oil recovery or bioremediation of these sites.

Method for pre-treatment of subterranean sites adjacent to water injection wells

A process for purifying 1,3-propanediol from the fermentation broth of a culturedthat has been bioengineered to synthesize 1,3-propanediol from sugar is provided. The basic process entails filtration, ion exchange and distillation of the fermentation broth product stream, preferably including chemical reduction of the product during the distillation procedure. Also provided are highly purified compositions of 1,3-propanediol.

Purification of biologically-produced 1,3-propanediol

Process for increasing the molecular weight of nylon 6 while reducing its content of caprolactam and other volatiles by a two-staged heating process.

Increased volatile removal during solid phase processing of nylon 6 by temperature programming

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Wilmington, DE, United States of America

Albert W Alsop