Semen Refinement B.v.

Stichting Centrum Voor Micro-elektronics Twente

Albert Van Den Berg

David N Reinhoudt

Adrianus J Sprenkels

Loes Irene Segerink

Peter D Van Der Wal

Wouter Olthuis

Ernst J Sudholter

Bjorn De Wagenaar

Maria D Skowronska-Ptasinska

The invention provides a system () for performing sperm analysis and selection based on sperm cell morphology of sperm cells () in a fluid (), the system () comprising: (i) a fluid flow channel () for transport of said fluid (), the fluid flow channel () comprising an inlet () an analyzing zone () configured downstream from said inlet () and comprising a first pair of electrodes () comprising a first intra-electrode distance (dl), a sorting zone () configured downstream from said analyzing zone () and comprising a sorting device (), and outlets (, . . . ) configured downstream from said sorting zone (); (ii) an electric source () configured to provide an electric signal to the first pair of electrodes (); (iii) a measuring device () functionally coupled to the first pair of electrodes () and configured to measure a first impedance as a function of time of the fluid () between the first pair of electrodes, and to provide time-dependent impedance data; wherein the sorting device () is configured to sort sperm cells () by directing the sperm cell () in the sorting zone () to one of the outlets (, . . . ) based on a comparison in a comparison stage of the time dependent impedance data with predefined reference data.

Microfluidic device for selection of semen

The invention relates to a process for manufacturing a REFET and/or CHEMFET, comprising: (a) covalent bonding of a hydrophilic polymer layer to an isolator layer applied to a semiconductor material; (b) the absorption of water or a watery solution into said hydrophilic polymer layer; and (c) the binding of a hydrophobic polymer layer to the water holding hydrophilic polymer layer. It is advantageous that this water holding hydrophilic polymer layer also contains an electrolyte and/or a buffer. Preferably an ion to be measured with the CHEMFET also forms part of the electrolyte.

Process for manufacturing a REFET or a CHEMFET, and the manufactured

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Enschede, Netherlands

Albert Van Den Berg