Boehringer Mannheim Gmbh

Albert Roder

Joachim Ziegenhorn

Hans Mollering

Hans Seidel

Joachim Siedel

Helmgard Gauhl

Walter Schneider

Helmut Lenz

Gunter Lang

Klaus Beaucamp

Rolf Deeg

Knut Bartl

Hans-Georg Batz

Wolfgang Gruber

Sigmar Klose

Gunter Weimann

Ulrich Neumann

Klaus Muhlegger

Herbert Jungfer

Peter Stahl

Friedrich Gotz

Gerhard Michal

Werner Stock

Detlef Von Hoerschelmann

Ellen Mossner

Karl-Heinz Schleifer

Erich Elstner

Ingeborg Gutmann

Karl-Wolfgang Knitsch

Harald Haug

Werner Kramer

Ulrich Nagele

Friedrich Giffhorn

Gunter Wehmeyer

Barbara Sedewitz

Rolf Laube

Robert C McCarthy

Werner Zwez

The present invention provides a process for the improved colorimetric determination of hydrogen peroxide as formed by a hydrogen peroxide-producing oxidase, by addition of a chromogenic system and measurement of the colored material formed, wherein superoxide dismutase (E.C. 1.15.1.1) is added to the reagent solution. The present invention also provides a reagent for the improved colorimetric determination of hydrogen peroxide, comprising a hydrogen peroxide-producing oxidase, a chromogenic system, a buffer and optionally adjuvant enzymes, wherein it also contains superoxide dismutase.

Process and reagent for the improved quantitative colorimetic

The invention teaches a method for improving the ability to determine a polyvalent substance via use of an immunoaggregate during the assay. The immunoaggregate eliminates substances which can lead to incorrect results otherwise.

Reagent and method for determination of a polyvalent substance using an

The present invention provides 1-methylhydantoinase, which hydrolyses 1-methylhydantoin in the presence of a nucleoside triphosphate and of polyvalent metal ions. The present invention also provides a process for obtaining 1-methylhydantoinase and a reagent containing it. Furthermore, the present invention provides a process for the determination of creatinine by the conversion of the creatinine with creatinine deiminase (E.C. 3.5.4.21) into 1-methylhydantoin, hydrolysis of the latter with the 1-methylhydantoinase in the presence of nucleoside triphosphate and of polyvalent metal ions and determination

Nucleoside triphosphate-dependent 1-methylhydantoinase, a process for

The present invention provides a process for the preparation of digitalis antibodies in which appropriate mammals are immunized with a digoxin bound to protein, the animal serum is obtained, the immune globulin-containing protein fraction is separated in known manner, the immunologically-active globulins are adsorbed on an immunologically-active column and separated from the other proteins, the antibodies are again eluted from the column and the Fab fractions are split with papain and purified, wherein, as immunologically-active adsorbent, there is used an inorganic matrix of large surface area to which digitoxin aldehyde is bound via a spacer which cannot be split with papain and the splitting off of the Fab fraction from the antibodies is carried out on the matrix. The present invention also provides digitalis antibodies obtained by this process, which antibodies can be used for the therapy of digitalis intoxications and for preparing immunological reagents for the determination of digitalis glycosides.

Digitalis antibodies, process for the preparation thereof and the use

The present invention provides a new pyruvate oxidase which decarboxylates pyruvate with the formation of hydrogen peroxide, characterized in that it is active without the addition of FAD, TPP and divalent metal ions. The present invention also provides a process for preparing this new enzyme and a reagent containing it.

Pyruvate oxidase

The present invention provides a process for the determination of N-carbamoylsarcosine, wherein a sample solution containing N-carbamoylsarcosine is reacted with N-carbamoylsarcosine-amidohydrolase to give sarcosine, which is then determined. The present invention also provides the enzyme N-carbamoylsarcosine-amidohydrolase, a process for obtaining it and a reagent containing it.

Process and reagent for the determination of N-carbamoylsarcosine with

A L(+)-tartrate dehydrogenase with L(+)-tartrate dehydrogenase and D(+)-malate dehydrogenase (decarboxylating) activity, which can be obtained from Rhodopseudomonas sphaeroides, and also a process for the production thereof. Furthermore, a process and a reagent for the determination of L(+)-tartrate and of D(+)-malate.

Determination of L(+)-tartrate or D(+)-malate with L(+)-tartrate

The present invention provides a process for the specific determination of the cholesterol of the LDL fraction in the presence of the HDL fraction of the lipoproteins of serum by the action of cholesterol esterase for the liberation of the cholesterol and oxidation of the liberated cholesterol with cholesterol oxidase and oxygen with the formation of hydrogen peroxide and cholestenone and kinetic measurement of the change of one of the reaction components of the oxidase reaction, especially the formation of hydrogen peroxide, wherein the measurement is carried out in a predetermined period of time, the reaction solution having a tenside concentration of 0.01 to 1.5 mmol/liter, a cholesterol esterase concentration of 0.1 to 30 U/ml. and a pH value of 6.5 to 8.0. The present invention also provides a reagent for carrying out this process.

Process for specific determination of cholesterol in the presence of the

The present invention provides a process for the determination of NAD(P)H or of salicylate, wherein, in a NAD(P)H-dependent reaction, salicylate is decarboxylated by salicylate hydroxylase and a colored material is formed from the decarboxylation product in the presence of tyrosinase by oxidative coupling with an appropriate colored material component, the colored material formed then being determined photometrically. The present invention also provides a reagent for the determination of NADH or NADPH, wherein it contains salicylate, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer, as well as a reagent for the determination of salicylate, wherein it contains NAD(P)H, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer.

Determination of NAD(P)H or salicylate

For the determination of glycerol, the latter is incubated with galactose oxidase in an aqueous medium in the presence of oxygen and either the oxygen consumption or the amount of H.sub.2 O.sub.2 or glyceraldehyde that is formed is determined. A reagent suitable for this purpose consists of galactose oxidase and a system for the determination of H.sub.2 O.sub.2 or a system for the determination of glyceraldehyde. It can additionally contain an agent for the saponification of esterified glycerol.

Method and reagent for the determination of glycerol

For the determination of glycerin by oxidation with oxygen in the presence of glycerinoxidase and measurement of the oxygen consumption or of the H.sub.2 O.sub.2 formation, a glycerinoxidase from Aspergillus spec. DSM 1729 is used. A reagent suitable for this method consists of glycerinoxidase from Aspergillus spec. DSM 1729 and a system for the determination of H.sub.2 O.sub.2 and contains additionally, if desired, an agent for the saponification of esterified glycerin.

Method and reagent for the determination of glycerin

A method for the determination of triglycerides by ester cleavage utilizing lipase and optionally esterase with the formation of fatty acids and glycerol, phosphorylation of the glycerol with adnosine triphosphate in the presence of glycerol kinase with the formation of glycerol-1-phosphate and adenosine diphosphate and determination of one of the latter two products. The glycerol kinase used is from Bacillus stearothermophilis and acts in combination with at least one activator selected from the group consisting of detergents, phenol derivatives and aniline derivative.

Method and reagent for the determination of triglycerides

A reconstitutable dry reagent for the turbidimetric determination of lipase, which reagent forms an emulsion upon adding water and comprises substrate oil, protective colloid, emulsifier and activator; and the process for making said dry reagent comprising preparing an aqueous emulsion of a triglyceride containing bile acid salt, colipase, at least 10% by weight of protective colloid, and at least a part of a preserving agent, lyophilizing the emulsion, mixing the lyophilizate with a buffer substance and urea, and recovering said dry reagent.

Reagent for the determination of lipase and process for preparing same

Formate, or compounds convertible into formate, is determined by reacting same in the presence of formate dehydrogenase (FDH) and of a hydrogen acceptor, e.g., NAD, wherein the formate dehydrogenase is from Candida boidinii DSM 941, to result in stoichiometric reactions. The process can be used for the removal of formate from solutions containing same.

Process for determining formate and reagent therefor

Stabilized urease compositions are provided comprising urease and, as a stabilizing agent, a mixture of glutathione, ethylenediamine-tetraacetic acid, and citrate, preferably in a weight ratio of 1:0.5-5:0.5-3:0.5-3.

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Seeshaupt, Germany

Albert Roder