Centre National De La Recherche Scientifique

Etablissement Francais Du Sang

Universitè De Montpellier

Universite Claude Bernard – Lyon1

University of Montpellier II

Girindus Ag

Centre National De La Recherche Scientifique-cnrs

L'université Montpellier II

Université De Montpellier 1

Albert Meyer

Jean-Jacques Vasseur

François Morvan

Carole Chaix

Carole Farre

Chantal Fournier-Wirth

Myriam Lereau

Julie Mayen

Jean-François Cantaloube

Eliane Souteyrand

Jean-Pierre Cloarec

Yann Chevolot

Sébastien Vidal

Jean-Louis Imbach

Cécile Dueymes

Fritz Link

Andreas Schönberger

Francois Morvan

Jean-Francois Cantaloube

Meinolf Lange

Françoise Debart

Ilaria Adamo

Andreas Schonberger

The present invention relates to a modified oligonucleotide having two or more thiol functions, which can be immobilized on a gold surface or on a grafted surface, in particular a surface comprising at least one carbon-carbon double bond or carbon-carbon triple bond or haloacetamide functions, preferably maleimide or acrylamide functions. The invention also relates to a method for detecting a nucleic acid in a biological sample comprising a step of detecting hybridization between a modified oligonucleotide and a target nucleic acid amplified from the biological sample. The invention relates more particularly to a method for detecting, genotyping or sequencing a pathogenic organism, preferably a virus.

Modified oligonucleotides comprising thiol functions and use thereof for detecting nucleic acids

The present invention relates to a thiol compound suitable for forming a chain of oligomers that can be grafted to an oligonucleotide. The invention also relates to an oligonucleotide grafted by such a compound, thus having one or more thiol functions, suitable for being immobilized on a gold surface or on a grafted surface.

Thiol compounds and the use thereof for the synthesis of modified oligonucleotides

A solution phase synthesis method for preparing an oligonucleotide, wherein at least some of the reagents are solid supported. The method suitable for large-scale synthesis comprises coupling a protected compound with a nucleotide derivative having a protection group in the presence of a solid supported activator to give an elongated oligonucleotide with a P(III)-internucleotide bond; optionally processing the elongated oligonucleotide by capping by reaction with a solid supported capping agent and/or by oxidizing or sulfurizing by reaction of the oligonucleotide with a solid supported oxidizing or sulfurization reagent; and removing the protection group. The coupling may include reacting a 3'-protected compound of formula: with a nucleotide derivative having a 5&#x2032;-protection group, or reacting a 5&#x2032;-protected compound of formula with a nucleotide derivative having a 3&#x2032;-protection group.

Method for preparing oligonucleotides

Method for the synthesis of nucleotide derivatives wherein molecules of interest are grafted on the oligonucleotide with the help of a 'click chemistry' reaction between an azide function on the molecule of interest and an alkyne function on the oligonucleotide, or between an alkyne function on the molecule of interest and an azide function on the oligonucleotide. Intermediate molecules, notably alkyne functionalized oligonucleotides, grafted oligonucleotides, azide functionalized oligonucleotides, oligonucleotide micro arrays containing them and the use of those grafted oligonucleotides for biological investigation and for cell targeting.

Method for the synthesis of oligonucleotide derivatives

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Perols, France

Albert Meyer