University of California

Becton Dickinson and Company Limited

Integrated Plasmonics Corporation

Albert Mach

Dino Di Carlo

Soojung C Hur

Smita Ghanekar

Marja Suni

Brian David Warner

David Vrane

Nilesh Dinbandhu Shah

Aaron J Middlebrook

Aspects of the present disclosure include methods for processing a biological sample. Methods according to certain embodiments include contacting a biological sample having cells with an assay reagent that includes one or more analyte-specific binding members to produce a biological sample assay composition and introducing the biological assay composition into an inlet of a flow cytometer having an integrated acoustic separator. Systems, including a flow cytometer with integrated acoustic separator having one or more acoustic concentrator devices suitable for practicing the subject methods are also described.

Multi-color flow cytometric analysis of samples with low cell numbers

A method of isolating cells includes providing a microfluidic device having at least one microfluidic channel coupled to an inlet and an outlet, the at least one microfluidic channel comprises at least one expansion region disposed along the length thereof. The at least one expansion region is an abrupt increase in a cross-sectional dimension of the at least one microfluidic channel configured to generate a vortex within the at least one expansion region in response to fluid flow. A solution containing a population of cells at least some of which have diameters &#x2265;10 &#x3bc;m flows into the inlet. A portion of cells is trapped within vortex created within the at least one expansion region. The trapped cells may then released from the expansion region.

Method and device for isolating cells from heterogeneous solution using microfluidic trapping vortices

Tissue dissociators configured to disrupt a biological tissue sample are provided. Aspects of the tissue dissociators may include a housing having a distal end and a proximal end, a cutting blade positioned at the distal end of the housing and a tissue actuator configured to be displaced along a longitudinal axis within the housing. Also provided are methods of using the tissue dissociators, as well as kits including the tissue dissociators.

Devices and methods for dissociating a biological tissue sample

A microcuvette cartridge for optical measurement of a specimen includes: a substrate having a recess on an upper surface thereof to receive a fluid specimen therein, the substrate having a plurality of cavities therein to receive the fluid specimen transported from the recess, the substrate further defining a plurality of channels communicating with the recess and with the plurality of cavities, respectively, to transport the fluid specimen from the recess to the plurality of cavities, said substrate further having one or more of windows at positions corresponding to the plurality of cavities, the windows being transparent to wavelength of light with which the optical measurement is to be carried out so as to allow the light to interact with the fluid specimen in the cavities; and a transport mechanism to promote and complete flows of the fluid specimen from the recess to the plurality of cavities through the plurality of channels.

Microcuvette cartridge

A method of isolating cells includes providing a microfluidic device having at least one microfluidic channel coupled to an inlet and an outlet, the at least one microfluidic channel comprises at least one expansion region disposed along the length thereof. The at least one expansion region is an abrupt increase in a cross-sectional dimension of the at least one microfluidic channel configured to generate a vortex within the at least one expansion region in response to fluid flow. A solution containing a population of cells at least some of which have diameters &#x2267;10 &#x3bc;m flows into the inlet. A portion of cells is trapped within vortex created within the at least one expansion region. The trapped cells may then released from the expansion region.

Growing community of inventors

San Jose, CA, United States of America

Albert Mach