Gist-brocades N.v.

Mogen International Nv

Albert Johannes Van Ooyen

Krijn Rietveld

Jacob B Visser

Wilhelmus J Quax

Andreas Hoekema

Peter Christiaan Sijmons

Jan Pen

Petrus Josephus Van Den Elzen

Hermanus C Kester

Johan A Van Den Berg

Claus Rolin

Yvonne Muller

Margo Anne-Rose Kusters-Van Someren

The present invention discloses a transformed Phaffia strain. Also disclosed are specific vectors for transforming Phaffia, preferably such vectors contain expression regulating sequences obtained from Phaffia. The present invention also discloses the use of certain marker genes in Phaffia. The invention further discloses a method for transforming Phaffia.

Transformation of phaffia rhodozyma

The exo-polygalacturonase gene from Aspergillus is identified, and the enzyme encoded thereby is expressed. Specifically, the exo-polygalacturonase gene from Aspergillus tubingensis is is cloned and expressed, and DNA sequences from other Aspergillus strains that specifically hybridize therewith are identified. Novel expression vectors that comprise an exo-polygalacturonase encoding sequence, and host cells transformed therewith, are also provided. The invention further relates to the production of recombinant exo-polygalacturonase, and to the use of this protein.

Cloning and expression of the exo-polygalacturonase gene from aspergillus

The present invention provides plants with a modified taste, solids content and/or texture. The invention also provides methods of obtaining such plants via transformation with DNA constructs containing genes encoding enzymes capable of degrading plant polysaccharides and optionally additional genes encoding enzymes which are capable of further modifying the degradation products resulting from the first degradation step.

Transgenic plants having a modified carbohydrate content

Kluyveromyces hosts and DNA expression cassettes for use in Kluyveromyces are provided for transcription of endogenous and/or exogenous DNA, and production of peptides, for enhancing production of an endogenous product, or producing an exogenous product. The Kluyveromyces hosts find particular use for secretion of a desired peptide product, where signal sequences may be native to the peptide or provided from endogenous or exogenous signal sequences, including synthetic sequences, functional in Kluyveromyces. A transformation procedure is provided for efficiently transforming Kluyveromyces.

Kluyveromyces as a host strain

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Voorburg, Netherlands

Albert Johannes Van Ooyen