Baxter Trading Gmbh

Dsm N.v.

Gist-brocades N.v.

Syngenta Mogen B.v.

Albert Johannes Joseph Van Ooyen

Krijn Rietveld

Hans Pannekoek

Robert Willem Van Leen

Martinus Philippus Verbeet

Wilhelmus J Quax

Andreas Hoekema

Jan Pen

Peter Christiaan Sijmons

Teunis Cornelis Verwoerd

Cornelis P Hollenberg

Sunil Das

Albert De Leeuw

Johan Abel Van Den Berg

A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture, especially a bakery process and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.

Production of enzymes in seeds and their use

Kluyveromyces hosts and DNA expression cassettes for use in Kluyveromyces are provided for transcription of endogenous and/or exogenous DNA, and production of peptides, for enhancing production of an endogenous product, or producing an exogenous product. The Kluyveromyces hosts find particular use for secretion of a desired peptide product, where signal sequences may be native to the peptide or provided from endogenous or exogenous signal sequences, including synthetic sequences, functional in Kluyveromyces. A transformation procedure is provided for efficiently transforming Kluyveromyces.

Kluyveromyces as a host strain

Novel polypeptides having Factor VIII activity are provided as well as compositions and methods for their preparation. The polypeptides comprise derivatives and fragments of Factor VIII and have sequences substantially similar to portions of naturally occuring Factor VIII. The polypeptides find use in treatment of Hemophilia A.

Proteins with factor VIII activity: process for their preparation using genetically-engineered cells and pharmaceutical compositions containing them

Novel polypeptides having Factor VIII activity are provided as well as compositions and methods for their preparation. The polypeptides comprise derivatives and fragments of Factor VIII and have sequences substantially similar to portions of naturally occuring Pactor VIII. The polypeptides find use in treatment of Hemophilia A.

Proteins with Factor VIII activity: process for their preparation using genetically-engineered cells and pharmaceutical compositions containing them

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Voorburg, Netherlands

Albert Johannes Joseph Van Ooyen