Aventis Pharma Deutschland, Gmbh

Novartis Corporation

Novartis Finance Corporation

Albert Hinnen

Hans-Friedrich Vahlensieck

Karl-Dieter Entian

Johannes Hegemann

Thomas Munder

Peter K&ouml;tter

Wilfried Kramer

Matthias Rose

Horst Feldmann

Friedrich Karl Zimmermann

Tillman Schuster

Method for screening antimycotically active substances

An assay to identify inhibitors of soraphen A resistant acetyl-coenzyme A carboxylase comprising measuring the reactivity of acetyl-coenzyme A carboxylase in the presence and in the absence of a compound suspected to inhibit soraphen A resistant acetyl-coenzyme A carboxylase reactivity, and comparing the reactivity measurements to identify inhibitors of soraphen A resistant acetyl-coenzyme A carboxylase.

Method for identifying inhibitors of soraphen A resistant

The present invention discloses DNA molecules comprising a gene encoding yeast acetyl-coenzyme A carboxylase resistant to soraphen A inhibition. Mutations confering soraphen A resistance to acetyl-CoA carboxylase can be dominant or recessive, and they can be point mutations, deletion or insertion mutations. In addition methods of isolating a gene encoding fungal acetyl-coenzyme A carboxylase resistant to soraphen A inhibition are provided as well as methods for purifying fungal acetyl-coenzyme A carboxylase resistant to soraphen A inhibition. Purified enzyme can be used in assays to identify inhibitors of soraphen A resistant acetyl-coenzyme A carboxylase.

Soraphen A resistant fungi and acetyl-CoA carboxylase

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Jena, Germany

Albert Hinnen