10x Genomics, Inc.

Albert Dale Kim

Meiliana Tjandra

Shalini Gohil

Joshua Delaney

Marlon Stoeckius

Yifeng Yin

Adam Lowe

Cedric Uytingco

Jennifer Chew

James Michael Chell

Jill Herschleb

Dulce Ovando Morales

Andrej Hartnett

Yi Luo

Kristen Pham

Monica Nagendran

Joshua Gu

Tathagata Mukherjee

James Francis Perna, Iii

Veronica Emelina Gonzalez Muñoz

The present disclosure relates in some aspects to methods and compositions for in situ analysis involving catalytic de-crosslinking of biological samples.

Catalytic de-crosslinking of samples for in situ analysis

The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.

Methods for generating barcoded nucleic acid molecules using fixed cells

Compositions and methods for using fixed biological samples

Provided herein are methods for preparing biological samples for spatial proteomic analysis, methods of determining a location of a protein analyte in a biological sample, and methods of determining a location of a protein analyte and a nucleic acid analyte in a biological sample.

Spatially-tagged analyte capture agents for analyte multiplexing

Growing community of inventors

Oakland, CA, United States of America

Albert Dale Kim