Quest Diagnostics Investments, Inc.

Albert Castro

Michelle M Tabb

Peter Lee

Lakshmi Nair

Maria Vestal

Lucien Jacky

Jose Matud

Provided herein are methods for determining the presence or absence of an enteroviruses and parechoviruses in a biological sample. The methods involve identifying the presence or absence of a target nucleic acids from the viruses using direct amplification from a biological sample without a step of extraction of the nucleic acids, but retaining substantially the same specificity and sensitivity of methods assaying extracted nucleic acids. Also provided are methods of diagnosis using the methods provided and compositions and kits for the practice of the methods.

Molecular detection of enterovirus and parechovirus

Disclosed are methods for identifying the presence or absence of a target nucleic acid from one or more organisms in a biological sample, said method comprising: (a) spinning the sample at a rotational velocity sufficient to pellet cellular debris and fluorescence inhibitors present within the sample and reduce fluorescence interference or quenching in the sample; and (b) directly amplifying and detecting the target nucleic acid in the sample.

Amplification and detection of nucleic acids in a biological sample

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Walnut, CA, United States of America

Albert Castro