Corning Glass Works

Becton Dickinson and Company Limited

Ciba Corning Diagnostics Corp.

Albert A Luderer

Ward C Smith

Nicholas Francis Borrelli

Anthony R Zine

Joseph N Panzarino

Gerald Odstrchel

Gerald R Mansfield

Richard J Carroll

Karen L Travis

Brenda D Manning

Hugh C McDonald

Michael A Harvey

Mary L Nicholson

This invention relates to an assembly for centrifugally separating lymphocytes and monocytes from the heavier phases in samples of human blood utilizing a liquid density gradient medium. The particular inventive subject matter concerns the use of novel partition means initially separating the liquid density gradient medium from the sample of blood and designs for such partition means.

Lymphocyte collection tube

A substantially pure antigen found on normal and benign breast epithelial cell membranes and in breast cancer cells, fused cell hybrids which produce antibodies specific for such antigen, the monoclonal antibodies produced by such fused cell hybrids, a method for detecting the presence of breast cancer in a patient which is based on measuring the concentrations of one or more determinants of such antigen in a patient sample, and a method for either identifying those breast cancer patients whose tumors would respond to estrogen manipulation or determining prognosis based on the degree of differentiation, which method is based on measuring the concentration of an estrogen-modulated determinant of such antigen.

Monoclonal antibodies specific for 330 KD breast tumor antigen and assay

This invention is directed to means for inhibiting the apparent shift in the buoyant density of and/or to restore any loss in the buoyant density of the granulocytic white blood cells in a sample of blood, thereby insuring the quality of the separation of lymphocytes and monocytes from granulocytes in a blood sample. The invention comprises contacting a blood sample with a hypertonic fluid containing a low molecular weight organic or inorganic ionic substance and/or with an isotonic or hypertonic fluid containing a high molecular weight organic substance which may contain organic molecules having a lipophilic substituent in their structures and/or with a culture medium for blood cells. An integral part of the invention is an improved blood separation tube utilizing a gel-like substance having a specific gravity between 1.060-1.065 g/cm.sup.3 to significantly enhance the purity of cell separation, while providing acceptable cell yields.

Separation of lymphocytes and monocytes from blood samples

This invention is directed to a method for reducing the mass of a tumor in animal tissue utilizing localized, magnetically-coupled, RF-induced hyperthermia. The method involves the implanting of a material in and/or closely adjacent to the tumor which is non-toxic to, and preferably inert to and compatible with, normal tissue and which has encapsulated therewithin ferromagnetic particles of such size, amount, composition, and ferromagnetism to develop a heating value of up to about one watt/gram, through essentially only hysteresis heating, under an applied field of about 20 but less than 200 oersteds at a frequency greater than 10 kilohertz and ranging up to about 600 kilohertz, or under an applied field of at least about 2000 oersteds and a frequency below about 40 hertz. Such heating value is sufficient to kill the tumor cells but muscle and nerve response of the animal body to the induced emf is minimized.

Radio frequency-induced hyperthermia for tumor therapy

The instant invention is directed to a noninvasive tumor treatment modality which results in a reduction of tumor mass and may lead to complete eradication of a tumor. The inventive method comprises localized magnetically-coupled, RF-induced hyperthermia mediated by a material which is non-toxic to and, preferably, compatible with animal tissue and has incorporated therewithin iron-containing crystals of such size, amount, composition, and magnetic properties to impart a coercive force of at least 200 oersteds to the material, and wherein the RF magnetic field has a frequency not in excess of about 10 kilohertz.

Radio frequency induced hyperthermia for tumor therapy

Obtaining a virus subpopulation, useful in qualitative and quantitative biological assay procedures, of enhanced sensitivity toward host cells for the virus by contacting a virus population with an inanimate substrate having moieties which mimic host cell receptor sites for cell binding protein of the virus and recovering as said subpopulation the portion of the initial virus population which complexes with the substrate.

Preparation of enriched whole virus radioligand

This invention relates to a method for isolating platelets, lymphocytes, and monocytes from anticoagulated blood. The method involves centrifuging at high force a sample of uncoagulated blood with a water-insoluble thixotropic gel-like material having a specific gravity between 1.065-1.077 g/cc for a length of time sufficient to cause the gel-like material to form a barrier between the platelets, lymphocytes, monocytes, and plasma fraction of the blood and the heavier blood cells. Thereafter, the plasma is withdrawn and the platelets, lymphocytes, and monocytes removed from above the barrier in a buffer solution. Where desired, the platelets can be separated from the lymphocytes and monocytes utilizing a similar process but wherein the water-insoluble thixotropic gel-like material has a specific gravity of less than about 1.055 g/cc.

Growing community of inventors

Marshfield, MA, United States of America

Albert A Luderer