Council of Scientific and Industrial Research

Alankar Arun Vaidya

Mohan Gopalkrishna Kulkarni

Bhalchandra Shripad Lele

Raghunath Anant Mashelkar

The present invention provides novel thermoprecipitating polymers of formula 1 containing novel enzyme sensitive ligands of the formula 4, processes for the preparation thereof respectively, and to the use thereof for the separation of enzymes. In formula 1 R is hydrogen or methyl, 1 is an integer in the range of from 1 to 10, m and n are either 0 or 1, x and y are integers greater than 1. 

Thermoprecipitating polymer containing enzyme specific ligands, process for the preparation thereof, and use thereof for the separation of enzymes

The present invention provides novel thermoprecipitating polymers of formula 1 containing novel enzyme sensitive ligands of the formula 4, processes for the preparation thereof respectively, and to the use thereof for the separation of enzymes. In formula 1 R is hydrogen or methyl, 1 is an integer in the range of from 1 to 10, m and n are either 0 or 1, x and y are integers greater than 1

The present invention relates to a process for the preparation of thermoprecipitating affinity polymers useful in the enhanced recovery of enzymes. The process comprises polymerising a monomer comprising a spacer and a co-monomer with a polymerisation initiator and a polymerisation accelerator at ambient temperature and pressure for a period ranging between 2 to 24 hours to obtain a polymer and then linking an inhibitor to pendant carboxyl groups of the spacers in the polymer to obtain an affinity polymer.

Process for the preparation of thermoprecipitating affinity polymers

The present invention provides novel thermoprecipitating polymers of formula 1, processes for the preparation thereof respectively and to the use thereof for the separation of enzymes. In formula 1 R is hydrogen or methyl, and x and y are integers greater than 1.

The present invention relates to a process for the preparation, of thermoprecipitating affinity polymers useful in the enhanced recovery of enzymes. The process comprises polymerising a monomer comprising a spacer and a co-monomer with a polymerisation initiator and a polymerisation accelerator at ambient temperature and pressure for a period ranging between 2 to 24 hours to obtain a polymer and then linking an inhibitor to pendant carboxyl groups of the spacers in the polymer to obtain an affinity polymer.

The invention relates to a process for the preparation of molecularly imprinted polymers useful for separation of enzymes, which comprises the steps of reacting a complex of enzyme and affinity monomer, a comonomer and a crosslinker, with a polymerization initiator and a polymerization accelerator at ambient temperature and pressure for a period ranging between 2 to 24 hrs, thereby obtaining a crosslinked polymer, crushing the cross linked polymer obtained to fine particles, adding a solvent and extracting imprinted enzyme from the polymer, obtaining the molecularly imprinted polymer, contacting the imprinted polymer with aqueous solution containing imprinted enzyme or a mixture of imprinted enzyme and other enzymes and isolating the enzyme-adsorbed polymer.

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Alankar Arun Vaidya