Vanderbilt University

Other

Firmenich Sa

Alan R Brash

Nathalie Tijet

William E Boeglin

Ian Michael Whitehead

Richard B Kim

Mitsuo Jisaka

Duncan James Horatio Gaskin

Ian M Whitehead

Urs W&auml;spi

Alan John Slusarenko

The present invention provides a fatty acid lyase, wherein the activity of the lyase for 9-hydroperoxide substrates is greater than the activity for 13-hydroperoxide substrates and wherein Kand Vof the lyase for 9-hydroperoxylinolenic acid are greater than Kand Vof the lyase for 9-hydroperoxylinoleic acid. More particularly, the invention provides a lyase present in melon (). The invention also provides a nucleic acid encoding the lyase, vectors, and expression systems with which the recombinant lyase can be obtained. The invention also provides methods of using the lyase of the invention, including methods of cleaving 9-hydroperoxylinoleic acid, 9-hydroperoxylinolenic acid, 13-hydroperoxylinoleic acid, and 13-hydroperoxylinolenic acid. Also, the invention provides a method of preparing 3-(Z)-nonenal, (3Z,6Z)-nonadienal, 2-(E)-nonenal, (2E,6Z)-nonadienal, or their corresponding alcohols and a method of preparing n-hexanal, 3-(Z)-hexen-1-al, 2-(E)-hexen-1-al, or their corresponding alcohols using the lyase of the present invention.

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The present invention relates to fatty acid 13-hydroperoxide lyase protein from guava ( ) and the gene encoding the protein. Expression systems for recombinant guava 13-hydroperoxide lyase and methods of using recombinant guava 13-hydroperoxide lyase for the production of green notes are provided.

Guava (Psidium guajava) 13-hydroperoxide lyase and uses thereof

Isolated and purified lipoxygenase proteins and nucleic acids are described. Particularly, a novel human 15(S) lipoxygenase (15-Lox-2) protein and cDNA and a cDNA for mouse 8S-lipoxygenase are described. Recombinant host cells, recombinant nucleic acids and recombinant proteins are also described, along with methods of producing each. Isolated and purified antibodies to 15-Lox-2 and 8-Lox, and methods of producing the same, are also described.

Lipoxygenase proteins and nucleic acids

Isolated and purified lipoxygenase proteins and nucleic acids are described. Particularly, a novel human 12R-lipoxygenase (12R-LO) protein and cDNA are described. Recombinant host cells, recombinant nucleic acids and recombinant proteins are also described, along with methods of producing each. Isolated and purified antibodies to 12R-LO, and methods of producing the same, are also described.

Isolated and purified 12R-lipoxygenase protein and nucleic acids

The present invention provides a fatty acid lyase, wherein the activity of the lyase for 9-hydroperoxide substrates is greater than the activity for 13-hydroperoxide substrates and wherein K,and V,of the lyase for 9-hydroperoxylinolenic acid are greater than K,and V,of the lyase for 9-hydroperoxylinoleic acid. More particularly, the invention provides a lyase present in melon (,). The invention also provides a nucleic acid encoding the lyase, vectors, and expression systems with which the recombinant lyase can be obtained. The invention also provides methods of using the lyase of the invention, including methods of cleaving 9-hydroperoxylinoleic acid, 9-hydroperoxylinolenic acid, 13-hydroperoxylinoleic acid, and 13-hydroperoxylinolenic acid. Also, the invention provides a method of preparing 3-(Z)-noncnal, (3Z,6Z)-nonadienal, 2-(E)-nonenal, (2E,6Z)-nonadienal, or their corresponding alcohols and a method of preparing n-hexanal, 3-(Z)-hexen-1-al, 2-(E)-hexen-1-al, or their corresponding alcohols using the lyase of the present invention.

Muskmelon (Cucumis melo) hydroperoxide lyase and uses thereof

The present invention relates to fatty acid 13-hydroperoxide lyase protein from guava (,) and the gene encoding the protein. Expression systems for recombinant guava 13-hydroperoxide lyase and methods of using recombinant guava 13-hydroperoxide lyase for the production of green notes are provided.

Growing community of inventors

Nashville, TN, United States of America

Alan R Brash