Sangamo Biosciences, Inc.

Sangamo Therapeutics, Inc.

Alan P Wolffe

Fyodor Urnov

Trevor Collingwood

Edward J Rebar

Qiang Liu

Stephen P Eisenberg

Eric E Jarvis

Andrew Jamieson

Pei-Qi Liu

Dmitry Y Guschin

Michael Moore

Elizabeth J Wolffe, Legal Representative

Timothy Farries

Xiao-Yong Li

Brian Johnstone

Philip D Gregory

Andrew Snowden

Casey Christopher Case

Eva Raschke

Methods and compositions for modifying stem cells using one or more ZFPs are disclosed. Such methods and compositions are useful for facilitating processes such as, for example, dedifferentiating cells, differentiating stem cells into the desired phenotype, propagating stem cells and/or facilitating cloning.

Modulation of stem cells using zinc finger proteins

Methods and compositions for modifying stem cells using one or more ZFPs are disclosed. Such methods and compositions are useful for facilitating processes such as, for example, dedifferentiating cells, differentiating stems cells into the desired phenotype, propagating stem cells and/or facilitating cloning.

Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.

Regulation of angiogenesis with zinc finger proteins

Methods and compositions for targeted modification of chromatin structure, within a region of interest in cellular chromatin, are provided. Such methods and compositions are useful for facilitating processes such as, for example, transcription and recombination, that require access of exogenous molecules to chromosomal DNA sequences.

Targeted modification of chromatin structure

Methods and compositions for the identification, isolation and characterization of regulatory DNA sequences in a cell of interest are provided. In particular, libraries of regulatory sequences are provided, in which each member of the library comprises a polynucleotide comprising sequences from an accessible region of cellular chromatin.

Libraries of regulatory sequences, methods of making and using same

Methods and compositions for the identification, isolation and characterization of regulatory DNA sequences in a cell of interest are provided. Also provided are libraries of regulatory sequences obtained according to the methods, and databases comprising collections of regulatory sequences for a particular cell of interest. In addition, various uses for the regulatory sequences so obtained, and uses for the databases of regulatory sequences, are provided. Also disclosed are computer systems and computer program products for utilizing the databases to conduct various genetic analyses, and uses of accessible regulatory sequences in the design of vectors bearing transgenes.

Databases of regulatory sequences; methods of making and using same

Methods for assessing potential pharmacological effects and/or side effects of an agent, based on the effect of the agent on the distribution and properties of accessible regions in cellular chromatin, are provided.

Screening methods based on isolating a collection of polynucleotides corresponding to accessible regions of chromatin

Methods for the identification of a drug target, wherein the drug target is a component of a signal transduction pathway, are provided. The methods are based, in part, on selection of a gene of therapeutic interest and determination of regulatory DNA sequences which regulate expression of the gene in a cell of interest, followed by reconstruction of signal transduction pathways which act on molecules which bind to the regulatory sequence. Also provided are methods for assessing potential pharmacological effects and/or side effects of an agent, based on the effect of the agent on the distribution and properties of accessible regions in cellular chromatin.

Pharmacogenomics and identification of drug targets by reconstruction of signal transduction pathways based on sequences of accessible regions

Methods for the design of exogenous regulatory molecules, comprising a DNA-binding domain and a functional domain, are provided. The methods rely on the identification, isolation and characterization of regulatory DNA sequences obtained on the basis of their accessibility in cellular chromatin.

Growing community of inventors

Orinda, CA, United States of America

Alan P Wolffe