Novozymes, Inc.

Novo Nordisk Biotech, Inc.

Other

Novo Nordisk A/s

Valent Biosciences Corporation

Novozymes Biotech, Inc

Abbott Laboratories Corporation

Novozymes A/s

Japan Tobacco Inc.

Novozymes Biopharma Dk A/s

Alan P Sloma

Debbie Sue Yaver

William R Widner

Maria Tang

David Sternberg

Michael David Thomas

Regine Behr

Lee Fremont Adams

Stephen H Brown

Helle Outtrup

Claus Dambmann

Dorrit A Aaslyng

Linda Sternberg, Legal Representative

Kimberly M Brown

Alexander Blinkovsky

Alan V Klotz

Tony S Byun

Mikio Fujii

Chigusa Marumoto

Leslie Naggiar, Legal Representative

Lene Venke Kofod

Steen Troels J&oslash;rgensen

Per Lin&aring; J&oslash;rgensen

B&oslash;rge Krag Diderichsen

Michael Thomas

Steen Troels Jo Slashedrgensen

Bo Slashedrge Diderichsen

Lynne M Christianson

Per Linang Jo Slashedrgensen

Stephen Brown

William Widner

Michael D Thomas

The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating ahost cell under conditions suitable for production of the hyaluronic acid, wherein thehost cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene. The present invention also relates to isolated nucleic acid sequences encoding a UDP-glucose 6-dehydrogenase, UDP-glucose pyrophosphorylase, and UDP-N-acetylglucosamine pyrophosphorylase.

Methods for producing hyaluronan in a recombinant host cell

The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parentcell under conditions conducive for the production of the heterologous biological substance, wherein (i) the mutant cell comprises a first nucleic acid sequence directing synthesis of the heterologous biological substance and a second nucleic acid sequence comprising a modification of at least one of the genes cypX and yvmC, which are involved in the production of a red pigment, and (ii) the mutant cell is deficient in the production of the red pigment compared to the parentcell when cultivated under the same conditions; and (b) recovering the heterologous biological substance from the cultivation medium. The present invention also relates to mutants ofcells and methods for producing the mutants.

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The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating ahost cell in a medium conducive for the production of the polypeptide, wherein thecell comprises a nucleic acid construct comprising (i) a tandem promoter in which each promoter sequence of the tandem promoter is operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and alternatively also (ii) an mRNA processing/stabilizing sequence located downstream of the tandem promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to methods for producing a polypeptide, comprising: (a) cultivating ahost cell in a medium conducive for the production of the polypeptide, wherein thecell comprises a nucleic acid construct comprising (i) a 'consensus' promoter having the sequence TTGACA for the '&#x2212;35' region and TATAAT for the &#x201c;&#x2212;10&#x201d; region operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and (ii) an mRNA processing/stabilizing sequence located downstream of the &#x201c;consensus&#x201d; promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium.

The present invention relates to isolated polypeptides having aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.

Polypeptides having aminopeptidase activity and nucleic acids encoding same

The invention relates to a method for producing an integrant(s) of,which produces a larger quantity of a crystal delta-endotoxin with greater pesticidal activity as compared to the crystal delta-endotoxin produced by the corresponding parental strain. The crystal delta-endotoxin produced by the integrant,will have an activity directed towards the same pest(s) as its parent,crystal delta-endotoxin. The invention further relates to such integrants, compositions comprising such integrants, as well as methods for controlling a pest(s) using these compositions.

Formation of and methods for the production of large bacillus thuringiensis crystals with increased pesticidal activity

The invention relates to a method for producing an integrant(s) of,which produces a larger quantity of a crystal delta-endotoxin greater pesticidal activity as compared to the crystal delta-endotoxin produced by the corresponding parental strain. The crystal delta-endotoxin produced by the integrant,will have an activity directed towards the same pest(s) as its parent,crystal delta-endotoxin. The invention further relates to such integrants, compositions comprising such integrants, as well as methods for controlling a pest(s) using these compositions.

Formation of and methods for the production of large Bacillus thuringiensis crystals with increased pesticidal activity

The invention relates to a method for producing an integrant(s) of,. The invention further relates to such integrants, compositions comprising such integrants, as well as methods for controlling a pest(s) using these compositions.

Production of Bacillus thuringiensis integrants

The invention relates to a method for producing an integrant(s) of,The invention further relates to such integrants, compositions comprising such integrants, as well as methods for controlling a pest(s) using these compositions.

The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a Bacillus host cell in a medium conducive for the production of the polypeptide, wherein the Bacillus cell comprises a nucleic acid construct comprising (i) a tandem promoter in which each promoter sequence of the tandem promoter is operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and alternatively also (ii) an mRNA processing/stabilizing sequence located downstream of the tandem promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to methods for producing a polypeptide, comprising: (a) cultivating a Bacillus host cell in a medium conducive for the production of the polypeptide, wherein the Bacillus cell comprises a nucleic acid construct comprising (i) a &ldquo;consensus&rdquo; promoter having the sequence TTGACA for the &ldquo;&minus;35&rdquo; region and TATAAT for the &ldquo;&minus;10&rdquo; region operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and (ii) an mRNA processing/stabilizing sequence located downstream of the &ldquo;consensus&rdquo; promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium.

Methods for producing a polypeptide in a Bacillus cell

Growing community of inventors

Davis, CA, United States of America

Alan P Sloma