University of Arkansas

University of Georgia Research Foundation Inc

Embrex, Inc.

Alan P Avakian

Craig E Whitfill

John A Thoma

Eid E Haddad

Stanley H Kleven

Vivian W Doelling

Cherilyn L Heggen-Peay

A method of producing active immunity against a bacterial or protozoal disease in a subject comprises administering to the subject a vaccine conjugate comprising a live bacteria or protozoa and a neutralizing factor bound to the live bacteria or protozoa. The neutralizing factor is selected from the group consisting of antibodies and antibody fragments. The live bacteria or protozoa is one capable of producing disease in the subject, and the antibody or antibody fragment is one capable of neutralizing the live bacteria or protozoa.

Methods of treatment

Method of treatment

The present invention relates to the identification and purification of antigens from M. gallisepticum and M. synoviae and their use in serological assays. Cell proteins from these organisms that possess substantial immunoreactive responses with poultry antisera to the mycoplasma from which they are isolated in both the acute and convalescent stages of infection are identified. Of these immunogenic proteins, those that also lack immunogenic cross-reactivity with antisera to other poultry mycoplasmas and pathogens are identified. These species specific proteins are purified and incorporated into enzyme-linked immunosorbent assays (ELISAs). The resulting assays are able to detect sensitively and selectively both field or experimentally-induced M. gallisepticum or M. synoviae infection in poultry. A combination of these assays are incorporated into a diagnostic kit to allow the rapid, sensitive, and selective testing for and differentiation between M. gallisepticum and M. synoviae. This invention also provides anti-serum to these mycoplasma antigens and an immunoassay that employs this anti-serum to detect infection in poultry. Furthermore, a method to produce genetic clones that express the antigenic portions of the mycoplasma antigens is provided.

Method of detecting mycoplasma infection in poultry and compositions

Growing community of inventors

Raleigh, NC, United States of America

Alan P Avakian