E.i. Dupont De Nemours and Company

Dade Chemistry Systems Inc.

Alan M Hochberg

James E Davis

Alan Robert Craig

Steven J Zoha

James M Prober

Rudy J Dam

A method is disclosed for determining the sequence of DNA bases by using two electrophoretic lanes for each DNA sample to be sequenced. Each lane incorporates two dyes.

Method for determining DNA sequences

This invention uses the evanescent wave detection of particles to distinguish bound from free in an analyte-binding assay. Illumination below the critical angle is employed, and a beveled window is used to eliminate bulk fluorescence from the emitted evanescent wave liquid. The sample is held in a non-rigid film cuvette.

Specific binding assay involving separation of light emissions

Apparatus for performing fluorescent assays which separates bulk and

The method of this invention obtains the underlying peak shapes of two signal spectrums whose ratios are representative of different species. The underlying peak shapes are fitted to the signal spectrums in a way that yield accurate ratios. The ratios are accurate even when the location of the peaks are not precisely known.

Growing community of inventors

Hockessin, DE, United States of America

Alan M Hochberg