Centenary Institute of Cancer Medicine and Cell Biology

The Macfarlane Burnet Institute for Medical Research and Public Health Limited

Alan Lee Landay

Anthony Dominic Kelleher

John James Zaunders

Nabila Seddiki

Barbara Denise Fazekas De St Groth

Sarah Christina Sasson

David Andrew Anderson

Mary Louise Garcia

Robyn Elizabeth Lloyd

Suzanne Mary Crowe

The present invention relates to methods and kits for identifying, quantifying and isolating regulatory T cells, to methods and kits for diagnosing or monitoring autoimmune diseases, immunoinflammatory diseases, allergic diseases, predispositions thereto, infectious diseases, cancer, cancer treatment and/or organ transplantation based on regulatory T cell quantity, to methods and kits for predicting responses to therapy for autoimmune diseases, immunoinflammatory diseases, allergic diseases, predispositions thereto, infectious diseases, cancer and/or organ transplantation based on regulatory T cell quantity, and to methods and kits for therapy using isolated regulatory T cells.

Kit for identifying regulatory T cells

The invention provides kits and methods for detecting or monitoring the number of cells in sample. The cell comprises a cell surface associated protein (CSAP) comprising a cytoplasmic (cytosolic) and an extracellular (ecto) domain. The kit comprises: (i) a chromatographic device; and (ii) a CSAP-binding agent. The method comprises: (i) optionally contacting the sample with an agent capable of lysing or permeabilizing CSAP bearing cells; (ii) contacting the sample with a CSAP-binding agent that binds to the cytoplasmic domain of the CSAP; and (iii) directly or indirectly evaluating the level or presence of bound CSAP in the sample.

Method of diagnosis and kit therefor

Method for identifying regulatory T cells

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River Forest, IL, United States of America

Alan Lee Landay