Brigham Young University

University of Utah Research Foundation

Leco Corporation

Arup Laboratories, Inc.

Sensar, Inc.

Alan L Rockwood

Edgar D Lee

Milton L Lee

Mark M Kushnir

A Wayne Meikle

Randall W Waite

Stephen A Lammert

Bingfang Yue

Jeffrey L Jones

Larry J Davis

H Dennis Tolley

James Oliphant

Samuel E Tolley

Nosa Agbonkonkon

Gordon J Nelson

Li Zhou

A method of separating free target analyte from protein-bound target analyte is described. Such can include obtaining an aqueous sample containing a target analyte in a free form (free target analyte) and the target analyte in a protein-bound form (protein-bound target analyte), passing the aqueous sample through a size exclusion chromatography matrix with a molecular weight cut off sufficient to allow the free target analyte to permeate into pores of the size exclusion chromatography matrix and exclude the protein-bound target analyte, whereupon the free target analyte adheres to and is immobilized by the size exclusion chromatography matrix and the protein-bound target analyte does not adhere to by the size exclusion chromatography matrix, separating the free target analyte from the protein-bound analyte by removing the protein-bound target analyte from the size exclusion chromatography matrix, and eluting the free target analyte from the size exclusion chromatography matrix with an organic solvent.

Separating and quantifying unbound target analytes from biological samples

The present invention provides methods, compositions, and kits associated with analyzing, enriching, and/or isolating a biomarker or analyte in a biological sample. In one aspect, for example, a method for determining a concentration of a biomarker in a biological sample can include binding any unbound biomarker with an antibody specific for the biomarker to form antibody-bound biomarker, enriching the antibody-bound biomarker and any endogenous autoantibody-bound biomarker to form an enriched fraction, identifying the biomarker in the enriched fraction, and determining the concentration of the biomarker in the biological sample. In one aspect, the concentration of the biomarker is derived from initially unbound biomarker and autoantibody-bound biomarker in the biological sample.

Methods for analysis of free and autoantibody-bound biomarkers and associated compositions, devices, and systems

Methods for measuring and analyzing parathyroid hormone-related peptide (PTHrP) using LC-MS/MS, including applications of the methods thereof, are disclosed and discussed. Such methods can include, along with the use of an isotope-labeled internal standard, purifying PTHrP from a biological sample, proteolytically digesting the PTHrP, and measuring specific digestion products using the using LC-MS/MS.

High sensitivity measurement of parathyroid hormone-related peptide using LC-MS/MS and associated methods

The present disclosure provides methods and composition involving increased sensitivity of compounds using mass spectrometry. In one embodiment, a method of increasing the sensitivity for detection of a carbonyl group-containing compound by mass spectrometry can comprise derivatizing the carbonyl group-containing compound with an O-substituted hydroxylamine thereby producing an oxime, resulting in enhanced sensitivity of detection by mass spectrometry, as compared to the underivatized carbonyl group-containing compound. Additionally, a method for assaying a carbonyl group-containing compound can comprise reacting the carbonyl group-containing compound in a sample with an O-substituted hydroxylamine to produce an oxime and performing analysis with mass spectrometric detection of the oxime by a mass spectrometry instrument. Further, an assay for a ketosteroid can comprise a derivatized ketosteroid produced by reacting the ketosteroid with an O-substituted hydroxylamine to produce an oxime and a mass spectrometry instrument for performing mass spectrometric analysis on the derivatized ketosteroid.

Enhanced sensitivity for analysis of ketosteroid compounds using mass spectrometry

A virtual ion trap that uses electric focusing fields instead of machined metal electrodes that normally surround the trapping volume, wherein two opposing surfaces include a plurality of uniquely designed and coated electrodes, and wherein the electrodes can be disposed on the two opposing surfaces using plating techniques that enable much higher tolerances to be met than existing machining techniques.

Virtual ion trap

A cross-flow ion mobility analyzer (CIMA) that includes a component of gas flow that opposes an electric field that is established within a channel, wherein ions are carried through the channel, wherein ions of a specific mobility are trapped by the opposing electric field and flow field within the channel and are detected when the ions reach the end of the channel, wherein a detector at the end of the channel sees a continuous stream of mobility-selected ions, and wherein different ions are selected by modifying the electric field and/or the velocity of the flow field.

Cross-flow ion mobility analyzer

The present invention provides methods for high throughput analysis of analytes in complex mixtures for unresolved chromatographic peaks including specific embodiment for summing intensities for each mass transition of interest over a selected chromatographic peak () to generate a signal corresponding to total intensity for each transition (). The intensities are deconvoluted into intensities of individual analytes (), based on branching ratios acquired from authentic standards, and a comparison to calibration curve is performed to obtain a quantitative concentration measurement of a particular analyte in a sample.

Methods for quantitative analysis by tandem mass spectrometry

A single set of electrodes, wherein different electrical potentials are applied to the single set of electrodes at different times in order to perform both ion mobility-based spectrometry and mass spectrometry (MS) on a sample of ions, wherein the ions are processed by performing ion mobility-based spectrometry and mass spectrometry in any sequence, any number of times, and as isolated or superposed procedures in order to trap, separate, fragment, and/or analyze charged particles and charged particles derived from atoms, molecules, particles, sub-atomic particles and ions.

Single device for ion mobility and ion trap mass spectrometry

A system and method are provided of extracting spectra from data produced by temporally indexed spectral scans from a spectrometer and spectrometer. The method includes the operation of receiving a data matrix from the spectrometer. The noise can then be removed from the data matrix. A further operation is identifying spectra of interest in the data matrix based on information content. In addition, a reduction transformation can be applied to the data matrix based upon the spectra of interest based upon the denizen transformation for the purpose of extracting the spectra of interest from the data matrix.

System and method for extracting spectra from data produced by a spectrometer

A radio-frequency quadrupole ion guide having a symmetrical magnetic field disposed along an axis of the ion guide, wherein the system provides prolonged interaction between electrons and uncharged compounds within an ionization volume of the ion guide, resulting in enhanced ion creation.

Electron ionization source for othogonal acceleration time-of-flight mass spectrometry

A method and apparatus for focusing ions for delivery to an ion detection device using an aerodynamic ion focusing system that uses a high-velocity converging gas flow at an entrance aperture to focus an ion plume by reducing spreading and increasing desolvation of ions, and wherein a voltage is applied to at least a portion of the aerodynamic ion focusing system to assist in the focusing and delivery of ions to the ion detection device.

Method and apparatus for aerodynamic ion focusing

The present invention is embodied in a method and apparatus for utilizing inherently insulative material, such as the glass and epoxy resins used in construction of printed circuit boards, for construction of components in a time of flight mass spectrometer. By using printed circuit board materials, the present invention can take advantage of fabrication techniques for providing circuit traces on surfaces, from one side to another as a via, or embedded within the printed circuit material so as to be covered except where necessary to be exposed to make contact with other electrical devices. Embedded circuit traces also enable capacitive coupling to AC energy while maintaining electrical and physical isolation. Consequently, the present invention enables both invasive and non-invasive access to pressure and vacuum chambers.

Growing community of inventors

Provo, UT, United States of America

Alan L Rockwood