Dekalb Genetics Corporation

Monsanto Technology LLC

Other

Alan L Kriz

Michael H Luethy

Dale A Voyles

Paul S Chomet

Paul C Anderson

Matthew Curtis Griffor

Emil M Orozco, Jr

David McElroy

Matt Griffor

Shihshieh Huang

T Michael Spencer

Mathew Griffor

Methods and compositions for the expression of transgenes in monocot plants including maize are disclosed. In the invention, gene silencing is avoided by use of monocot-homeologous sequences from plants of the genusfor transformation. Included in these transgene sequences arepromoters, enhancers, coding sequences and terminators. Suitable alternatives to maize-derived transgenes are desirable for expression in maize in that homology-based gene silencing can limit or effectively eliminate transgene expression.

Methods and compositions for expression of transgenes in plants

The present invention provides a transgenic maize plant having in its genome transgenic DNA including sequence for zein reduction and sequence for lysine biosynthesis, whereby expression of the transgenic DNA results in a synergistically increased lysine content of seed of the transgenic maize plant. The invention further provides a method for providing maize seed with synergistically increased lysine content.

Maize seed with synergistically enhanced lysine content

The present invention provides a method for conferring tolerance to an amino acid analog of tryptophan to a plant and/or altering the tryptophan content of a plant by introducing and expressing an isolated DNA segment encoding an anthranilate synthase in the cells of the plant. Transgenic plants transformed with an isolated DNA segment encoding an anthranilate synthase, as well as seeds and progeny derived from these plants, are also provided. The present invention also provides a cDNA sequence of an alpha and a beta subunit of a maize anthranilate synthase.

Anthranilate synthase gene and method for increasing tryptophan production

The invention provides isolated gamma coixin promoters useful for the expression of heterologous coding sequences in transgenic plants. Further provided by the invention are transgenic plants comprising such promoters, expression constructs for the introduction of heterologous coding sequences into plants, and methods of using the plants and nucleic acids provided by the invention.

This invention provides an embryo specific promoter, e.g. derived from the 5' regulatory region of an emb5 gene. Compositions comprising this promoter include DNA constructs useful for plant transformation and plants transformed with such DNA constructs. Further provided are methods for the expression of transgenes in plants using the embryo specific promoter sequences. The methods of the invention include the direct creation of transgenic plants with the emb5 promoter by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

Maize embryo-specific promoter compositions and methods for use thereof

Methods and compositions for the expression of transgenes in monocot plants including maize are disclosed. In the invention, gene silencing is avoided by use of monocot-homeologous sequences from plants of the genus Coix for transformation. Included in these transgene sequences are Coix promoters, enhancers, coding sequences and terminators. Suitable alternatives to maize-derived transgenes are desirable for expression in maize in that homology-based gene silencing can limit or effectively eliminate transgene expression.

The current invention provides the maize A3 promoter and actin 2 intron. Compositions comprising these sequences are described, as well as transformation constructs derived therefrom. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the A3 promoter directly by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

Maize A3 promoter and methods for use thereof

Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction

The present invention provides methods and compositions for the identification of transgenic seeds. This is accomplished by use of screenable markers linked to aleurone-specific promoters. The screenable markers can be provided as gene fusions with selectable markers, allowing both selection and screening of transformants. The use of aleurone-specific promoters, which also direct expression in embryogenic tissues, allows efficient selection of transgenic cells and the screening of viable transgenic seeds, while avoiding the deleterious effects associated with constitutive expression of screenable marker genes. Screening of transgenic seeds avoids the need for growing and assaying of seeds for transgenes and allows implementation of automated seed screening techniques for the identification of transgenic seeds.

Growing community of inventors

Gales Ferry, CT, United States of America

Alan L Kriz