University of Texas System

Peregrine Pharmaceuticals, Inc.

Alan J Schroit

Krishnakumar Balasubramanian

Marya F McCarty

Philip E Thorpe

E Sally Ward Ober

Rajiv Nayar

Adi Gazdar

Shelley P M Fussey

Shelley Pm Fussey

Shelley PM Fussey

Disclosed are methods of identifying tumor-derived exosomes as an early cancer diagnostic, as well as for staging, assessing progression and assessing therapy of cancer.

Diagnostic test for early stage cancer

Disclosed are surprising new methods and compositions for isolating extracellular microvesicles such as exosomes, particularly disease-related and phosphatidylserine (PS)-positive extracellular microvesicles as exemplified by tumor- and viral-derived exosomes. The methods of the invention are rapid, efficient, cost-effective and, importantly, are suitable for use with large volumes of biological fluids and produce antigenically intact extracellular microvesicles and exosomes. The methods and compositions are based on the surprising use of acetate buffers to isolate large quantities of extracellular microvesicles, particularly tumor-derived exosomes, from solution, without damaging their morphological or functional properties or antigenicity.

Methods and compositions for isolating exosomes

The present disclosure provides a method of inhibiting angiogenesis within a tissue of interest by providing either intact or nicked &#x3b2;-Glycoprotein 1 (&#x3b2;2GP1) to cells associated with the tissue. The presence of &#x3b2;2GP1 inhibits angiogenesis within the tissue, in part by preventing neovascularization into the tissue. The disclosure also provides a method for treating tumors by providing &#x3b2;2GP1 to the tumor.

Beta-2-glycoprotein 1 is an inhibitor of angiogenesis

Disclosed are methods and compositions for the prevention and treatment of cancers using lipid-carrier protein conjugate compositions for generating lipid-specific immune responses in an animal. Also disclosed are methods for making phosphatidylserine conjugate compositions and their formulation for use in a variety of pharmaceutical applications including the detection and treatment of a variety of cancers and related conditions.

Methods and compositions for inducing autoimmunity in the treatment of cancers

The present disclosure provides a method of inhibiting angiogenesis within a tissue of interest by providing either intact or nicked &#x3b2;2-Glycoprotein 1 (&#x3b2;2GP1) to cells associated with the tissue. The presence of &#x3b2;2GP1 inhibits angiogenesis within the tissue, in part by preventing neovascularization into the tissue. The disclosure also provides a method for treating tumors by providing &#x3b2;2GP1 to the tumor.

&#x3b2;-2-glycoprotein is an inhibitor of angiogenesis

Liposome dispersions are prepared from (a) a synthetic phospholipid of the cephaline type in which the amino group is monoacylated with a dicarboxylic acid; (b) a synthetic phospholipid of the cephaline type; and (c) one or more compounds having pharmacological activity. The mixture optionally can contain phospholipids of natural sources and a pharmaceutical carrier solution buffered to pH 7.0 to 7.8.

Growing community of inventors

Houston, TX, United States of America

Alan J Schroit