University of Pittsburgh

Carnegie Mellon University

US Government As Represented by the Secretary of the Army

Agentase, LLC

Other

Bayer Corporation

Bayer Materialscience, LLC

Bayer Polymers LLC

Nanosembly, LLC

Biohybrid Solutions LLC

Alan J Russell

Eric John Beckman

Richard R Koepsel

Hironobu Murata

Krzysztof A Matyjaszewski

Chad S Cummings

William Richard Wagner

Douglas A Wicks

Karsten Danielmeier

Keith E LeJeune

Geraldine F Drevon

Jinyu Huang

William J Federspiel

Nicolay V Tsarevsky

Bhupendra P Doctor

Richard K Gordon

Donald M Maxwell

Ashima Saxena

Sang Beom Lee

David E Lenz

Michelle C Ross

Joel Kaar

Shawn R Feaster

Heung-Il Oh

Alan Enciso Barros

Liye Fu

Fotios M Andreopoulos

Nathaniel T Becker

Landon M Steele

Marina Kovaliov

Eliador J Ghenciu

Claire Komives

Apurva K Chaudhary

Diaf Abderrahmare

Christopher R Deible

Amanda Fisher

Keith Lejeune

Provided herein are materials and methods that include utilizing atom transfer radical polymerization (ATRP) initiator molecules that maintain a positive charge during biomacro-initiator synthesis.

Amino-reactive positively charged ATRP initiators that maintain their positive charge during synthesis of biomacro-initiators

Materials and methods for conducting an atom transfer radical polymerization in the presence of oxygen by interlocking enzymatic activities are provided herein.

Enzyme-assisted ATRP procedures

Protein-based affinity ligands, in which the protein has an affinity for a target protein and includes a covalently attached polyelectrolyte, and methods for using the same to purify proteins are described herein.

Affinity dissolution of biomolecules for protein purification

Embodiments of the invention provide at least one polymer covalently conjugated to an esterase. The at least one polymer includes a plurality of oxime functional groups.

Polymer engineered regenerating bioscavengers

Using a novel water-soluble, active ester amide-containing functionalized controlled radical polymerization initiator, stimuli responsive polymers have been grown from the surface of a protein, exemplified by chymotrypsin or any protein having surface amino acids that will covalently bind to the active ester amide-containing functionalized initiator. It is shown that changes in temperature or pH can change the conformation of the polymer surrounding the enzyme, which in turn enabled the rational tailoring of enzyme activity and stability. This method has afforded an increase in the activity and stability of the enzyme by an order of magnitude at pH's where the enzyme is usually inactive or unstable. Multimodal temperature responsive protein-block copolymer conjugates are described.

Polymer-based protein engineering methods to rationally tune enzyme activity, pH-dependence and stability

A polymer formed by controlled radical polymerization includes groups that can be modified after controlled radical polymerization to form a radical. The polymer can be the reaction product of a controlled radical polymerization of radically polymerizable monomers, wherein at least one of the radically polymerizable monomers includes at least one group that can be modified after the controlled radical polymerization to form a radical.

Modification of surfaces with polymers

A polymer formed by controlled radical polymerization includes groups that can be modified after controlled radical polymerization to form a radical. The polymer can be the reaction product of a controlled radical polymerization of radically polymerizable monomers, wherein at least one of the radically polymerizable monomers includes at least one group that can be modified after the controlled radical polymerization to form a radical. A compound includes a first group that is stimulated upon application of energy to the molecule to tether the molecule to a surface or to another polymer chain and a second group comprising a controlled radical polymerization initiator functionality. A block copolymer includes at least a first segment to impart a predetermined functionality to a target surface and at least a second segment including functional groups to interact with the targeted surface to attach the block copolymer to the surface. The first segment can free of functional groups that interact with the surface to attach the block copolymer to the surface.

A method of improving the blood compatibility of a blood-contacting surface includes immobilizing carbonic anhydrase on the surface, wherein the surface exhibits carbonic anhydrase activity of at least 20% of maximum theoretical activity of the surface based on monolayer surface coverage of carbonic anhydrase.

Devices, systems and methods for reducing the concentration of a chemical entity in fluids

A method for joining nanotubes end-to-end, includes contacting the nanotubes with a joining fluid. The joining fluid has at least one property that is different from at least one property associated with ends of the nanotubes. In one embodiment, the method further includes contacting the nanotubes with a first, treating fluid and removing the bulk first fluid before the nanotubes are contacted with the joining fluid. The joining fluid is immiscible in the treating fluid to effect end-to-end joining of the nanotubes.

End-to-end joining of nanotubes

A device for removal of at least a portion of carbon dioxide from an aqueous fluid includes at least one membrane through which carbon dioxide can pass to be removed from the fluid and immobilized carbonic anhydrase on or in the vicinity of a first surface of the membrane to be contacted with the fluid such that the immobilized carbonic anhydrase comes into contact with the fluid. The first surface exhibits carbonic anhydrase activity of at least 20% of maximum theoretical activity of the first surface of the membrane based on monolayer surface coverage of carbonic anhydrase in the case that the carbonic anhydrase is immobilize on the first surface.

The present invention provides amphiphilic diacetylene compounds, and compositions and self-assembled nanotubes containing the same. Also provided are methods of producing the compounds, compositions, and nanotubes of the invention, and methods of destroying or inhibiting the growth or proliferation of microorganisms using the nanotubes of the present invention.

Self assembled nanostructures and methods for preparing the same

A method of irreversibly immobilizing an enzyme in a polyurethane and an enzyme-containing polyurethane having a degree of immobilization of the enzyme of approximately 100%. The synthesis of waterborne polyurethanes in the presence of enzyme has enabled the irreversible attachment of the enzyme to the polymeric matrix. The distribution of immobilized enzyme as well as activity retention are homogeneous within the polyurethane. Decreasing ECC hydrophobicity, via the use of a less hydrophobic polyisocyanate prepolymer during polymerization, significantly enhanced the intrinsic activity of the ECC.

Irreversible immobilization of enzymes into polyurethane coatings

Growing community of inventors

Gibsonia, PA, United States of America

Alan J Russell