Cornell University

University of Nebraska

United States of America, as Represented by the Secretary of Agriculture

Kansas State University Research Foundation

Other

University of Nevada

Boyce Thompson Institute for Plant Research

The Institute for Genomic Research

Alan Collmer

James R Alfano

David W Bauer

Steven V Beer

Amy O Charkowski

Sheng-Yang He

Zhong-Min Wei

Ron J Laby

Samuel W Cartinhour

David J Schneider

Xiaoyan Tang

Adam J Bogdanove

Gregory B Martin

Sébastien Cunnac

C Robin Buell

Adela Ramos

Jong Hyun Ham

The present invention relates to methods of assembling a plurality of genetic units to form synthetic genetic constructs. This method involves appending universal adapter oligonucleotides and flexible adapter oligonucleotides to the 5' and 3&#x2032; ends of separate genetic units to be assembled to form separate dual extended genetic units. The dual extended genetic units are assembled together via homologous recombination between the flexible adapter oligonucleotide portions of the dual extended units to form synthetic genetic constructs. The present invention further relates to synthetic genetic constructs formed using the methods of the present invention, and vectors, cells, and organisms containing such synthetic genetic constructs.

Method of dual-adapter recombination for efficient concatenation of multiple DNA fragments in shuffled or specified arrangements

One aspect of the present invention relates to isolated nucleic acid molecules encoding avirulence proteins or polypeptides ofpv.DC 3000, or nucleic acid molecules which are complementary thereto. Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. Another aspect relates to the isolated proteins or polypeptides and compositions containing the same. The various nucleic acid molecules and proteins of the present invention can be used to impart disease resistance to a plant, make a plant hypersusceptible to colonization by nonpathogenic bacteria, modify a metabolic pathway in a cell, cause eukaryotic cell death and treat a cancerous condition, as well as inhibit programmed cell death.

Pseudomonas AVR and HOP proteins, their encoding nucleic acids, and use thereof

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The present invention relates to isolated nucleic acid molecules encoding a type III&#x2014;secreted bacterial protein capable of modifying a cell death pathway in a plant cell. One aspect of the present invention involves an isolated nucleic acid molecule having a nucleotide sequence that encodes the HopPtoD2 protein ofpv.DC 3000. Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. The nucleic acid molecules of the present invention can be used to impart disease resistance to a plant and to make a plant hypersusceptible to colonization by nonpathogenic bacteria.

Nucleic acids encoding pseudomonas hop proteins and use thereof

The present invention relates to isolated DNA molecules that encode proteins or polypeptides which elicit a hypersensitive response in plants. One aspect of the present invention involves an isolated DNA molecule that encodes the HopPtoP protein ofpv. tomato DC3000. The isolated DNA molecules can be used to impart disease resistance, stress resistance, and enhanced growth to plants or plants grown from treated seeds, to control insects on plants or plants grown from treated plant seeds, to impart post-harvest disease or desiccation resistance in fruits or vegetables, to impart enhanced longevity of fruit or vegetable ripeness, to impart desiccation resistance to cuttings of ornamental plants, and/or promote early flowering of ornamental plants, either by topical application of the proteins or polypeptides or transgenic expression in recombinant plants or plant seeds. Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are disclosed.

One aspect of the present invention relates to isolated nucleic acid molecules (i) encoding proteins or polypeptides ofCEL and EEL genomic regions, (ii) nucleic acid molecules which hybridize thereto under stringent conditions, or (iii) nucleic acid molecules that include a nucleotide sequence which is complementary to the nucleic acid molecules of (i) and (ii). Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. Another aspect relates to the isolated proteins or polypeptides and compositions containing the same. The nucleic acid molecules and proteins of the present invention can be used to imparting disease resistance to a plant, making a plant hypersusceptible to colonization by nonpathogenic bacteria, causing eukaryotic cell death, and treating cancerous conditions.

The present invention is directed to an a isolated DNA molecule fromthat encodes a protein or polypeptide which elicits a hypersensitive response in plants. This isolated DNA molecule can used to impart disease resistance to plants, to enhance plant growth, and/or to control insects on plants. Plants or plant seeds transformed with a DNA molecule encoding a hypersensitive response elicitor protein or polypeptide can be provided, and the transgenic plants or plants resulting from the transgenic plant seeds are grown under conditions effective to impart disease resistance, to enhance plant growth, and/or to control insects on plants or plants grown from the plant seeds.

DNA molecules and polypeptides of Pseudomonas syringae Hrp pathogenicity island and their uses

One aspect of the present invention relates to a DNA construct that contains a first DNA molecule encoding a functional type III secretion system, a promoter, and a second DNA molecule encoding a protein or polypeptide capable of being secreted by the type III secretion system. The second DNA molecule is operably coupled to the promoter so that upon introduction of the DNA construct into a host cell, the encoded protein or polypeptide and the type III secretion system are expressed and the encoded protein or polypeptide is secreted. Another aspect of the present invention relates to a system that includes (i) a first DNA construct having a first DNA molecule encoding a functional type III secretion system and (ii) a second DNA construct having a promoter operably coupled to a second DNA molecule encoding a protein or polypeptide capable of being secreted by the type III secretion system. Upon introduction of the first and second DNA constructs into a host cell, the encoded protein or polypeptide and the type III secretion system are expressed and the encoded protein or polypeptide is secreted. Methods of isolating a protein or polypeptide and identifying a gene encoding a potential effector protein or polypeptide are also disclosed.

Recombinant constructs and systems for secretion of proteins via type III secretion systems

The present invention relates to a method for delivering effector proteins into a target cell. This method involves introducing into the target cell an effector protein fused to a protein transduction domain of a human immunodeficiency virus TAT protein or derivatives or functional analogs thereof. The present invention also relates to a fusion protein including an effector protein fused to a protein transduction domain of a human immunodeficiency virus TAT protein or derivatives or functional analogs thereof. Another aspect of the present invention relates to a DNA construct including a first DNA molecule encoding an effector protein and a second DNA molecule operatively associated with the first DNA molecule and encoding a protein transduction domain of a human immunodeficiency virus TAT protein or derivatives or functional analogs thereof and its use in a method for delivering effector proteins into a target cell.

Recombinant constructs and techniques for delivering to eucaryotic cells bacterial proteins that are secreted via type III secretion systems

The nucleic acid and amino acid sequences for proteinaceous elicitors of the plant defense reaction known as the hypersensitive response are described along with methods for preparation and processes for inactivation.

Elicitor of the hypersensitive response in plants

The present invention is directed to an isolated protein or polypeptide which elicits a hypersensitive response in plants as well as an isolated DNA molecule which encodes the hypersensitive response eliciting protein or polypeptide. This isolated protein or polypeptide and the isolated DNA molecule can used to impart disease resistance to plants, to enhance plant growth, and/or to control insects on plants. This can be achieved by applying the hypersensitive response elicitor protein or polypeptide in a non-infectious form to plants or plant seeds under conditions effective to impart disease resistance, to enhance plant growth, and/or to control insects on plants or plants grown from the plant seeds. Alternatively, transgenic plants or plant seeds transformed with a DNA molecule encoding a hypersensitive response elicitor protein or polypeptide can be provided and the transgenic plants or plants resulting from the transgenic plant seeds are grown under conditions effective to impart disease resistance, to enhance plant growth, and/or to control insects on plants or plants grown from the plant seeds.

Hypersensitive response elicitor from Pseudomonas syringae and its use

The present invention relates to an isolated protein or polypeptide corresponding to a protein or polypeptide in Erwinia chrysanthemi which elicits a hypersensitive response in plants. The encoding DNA molecule alone in isolated form or either in an expression system, a host cell, or a transgenic plant are also disclosed. Another aspect of the present invention relates to a method of imparting pathogen resistance to plants by transforming a plant with the DNA molecule of the present invention.

Hypersensitive response elicitor from Erwinia chrysanthemi

The nucleic acid and amino acid sequences for proteinaceous elicitors of the plant defense reaction known as the hypersensitive response against Pseudomonas syringae are described along with method for preparation.

Growing community of inventors

Ithaca, NY, United States of America

Alan Collmer