Dsm IP Assets B.v.

Cargill Inc.

Novozymes A/s

Novozymes, Inc.

Alan Berry

Debbie Sue Yaver

Holly J Jessen

Brian Jeffrey Rush

Michael Glenn Catlett

Beth M Mastel

Jeanette M Huryta

Michelle Barnhart

Anne Françoise Mayer

Masako Shinjoh

Stephen H Brown

Sheryl Luttringer

Rual Lopez-Ulibarri

Connie Lee

Werner Bretzel

Markus Hümbelin

Alexei A Yeliseev

Peter Bjarke Olsen

Steen Troels Joergensen

Torsten Bak Regueira

Bjarke Christensen

Petra Simic

Christian Manhart

Stephen Brown

Michelle Barnhardt

The present application discloses genetically modified yeast cells comprising an active 3-HP fermentation pathway, and the use of these cells to produce 3-HP.

Compositions and methods for 3-hydroxypropionic acid production

The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants.

Methods for improving malic acid production in filamentous fungi

The present invention relates to isolated protein complexes having succinyl-CoA:acetoacetate transferase activity, isolated polypeptide subunits thereof, and isolated polynucleotides encoding the subunits. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides and protein complexes.

Polypeptides having succinyl-CoA: acetoacetate transferase activity and polynucleotides encoding same

The present invention discloses an isolated polynucleotide molecule derived from a polynucleotide encoding a polypeptide having L-sorbosone dehydrogenase activity comprising a partial nucleotide sequence of at least 20 consecutive nucleotides of SEQ ID NO:1. The present invention further relates to a process for the production of L-ascorbic acid in high yield, in particular a process using resting cells of a microorganism able to convert given carbon sources into vitamin C. The thus obtained vitamin C may be further processed by purification and/or separation steps.

Microbial production of L-ascorbic acid

Polypeptides and encoding polynucleotides for microbial production of L-ascorbic acid and associated methods

The present invention relates to a process for the production of isoprenoids Coenzyme Q-10 by microorganisms. More particularly, the present invention relates to a process for increased production of Coenzyme Q-10 by microorganisms of the genus, preferably of the specieswhich have been transformed with one or more gene(s) of the mevalonate (mev) operon from a different microorganism, preferably of the genus, more preferably of the species, whereby the mev operon is mutated leading to an increased coenzyme Q-10 production. Sequences carrying such a mutation as well as a microorganism carrying such a mutated mev operon are also included.

Production of isoprenoids

Isolated polynucleotides encoding polypeptides having the activity of enzymes in the mevalonate pathway are provided. These sequences are useful for recombinantly producing isoprenoid compounds, such as carotenoids, in particular zeaxanthin. Expression vectors, cultured cells, and methods of making isoprenoid compounds are also provided.

Isoprenoid production

Growing community of inventors

Granite Bay, CA, United States of America

Alan Berry