Biogen Ma Inc.

Lallemand Hungary Liquidity Management LLC

University of Minnesota

Stellenbosch University

Gloriana Therapeutics, Inc.

Universiteit Stellenbosch

Alan Benjamin Gilbert

Rashmi Rohit Kshirsagar

Vineet Rajgarhia

John E McBride

Allan C Froehlich

Haowen Xu

Merja Elisa Penttila

Charles F Rice

Matti Siika-Aho

Elena E Brevnova

Mark Mellon

Anne K Warner

Marja Ilmen

Emily Stonehouse

Riaan Den Haan

Kristen M Deleault

John S Bardsley

Indraneel Shikhare

Sanni Voutilainen

Jaana Uusitalo

Anu Koivula

Deidre Willes

Aaron Argyros

Arthur J Shaw, Iv

Friedrich Srienc

William Ryan Sillers

David Anthony Hogsett

Thomas Ryll

Emile Van Zyl

Kyle McElearney

Danie Lagrange

Lia Tescione

Elena Brevnova

Jim Flatt

Cong Trinh

Steve Licht

Hans Van Dijken

Naomi Thorngren

Emily A Stonehouse

Dan Murphy

Heidi Hanson Hau

Jeffrey D Keating

Alan Froehlich

Terrence Michael Dobrowsky

Pornkamol Unrean

Shaunita Rose

Jeff Villari

Chhayal Ghandi

Merja Penttila

Arthur J Shaw Iv

Vineet B Rajgarhia

John E Mcbride

The present invention pertains to a cell culture medium comprising manganese as a media supplement, which was shown to control recombinant protein glycosylation and methods of using thereof. The present invention further pertains to a method of controlling or manipulating glycosylation of a recombinant protein of interest in a large scale cell culture.

Manganese supplementation for control of glycosylation in mammalian cell culture process

The present disclosure pertains to a mammalian cell culture genetically modified to express, and which expresses, a neublastin antibody polypeptide, or fragment thereof, in the culture, and to a neublastin antibody polypeptide, or fragment thereof, made by a mammalian cell culture genetically modified to express, and which expresses, the neublastin antibody polypeptide, or fragment thereof.

Mammalian cell culture-produced neublastin antibodies

The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.

Methods of preventing and removing trisulfide bonds

The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterologous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability ofto produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.

Yeast expressing cellulases for simultaneous saccharification and fermentation using cellulose

One aspect of the invention relates to a genetically modified thermophilic or mesophilic microorganism, wherein a first native gene is partially, substantially, or completely deleted, silenced, inactivated, or down-regulated, which first native gene encodes a first native enzyme involved in the metabolic production of an organic acid or a salt thereof, thereby increasing the native ability of said thermophilic or mesophilic microorganism to produce lactate or acetate as a fermentation product. In certain embodiments, the aforementioned microorganism further comprises a first non-native gene, which first non-native gene encodes a first non-native enzyme involved in the metabolic production of lactate or acetate. Another aspect of the invention relates to a process for converting lignocellulosic biomass to lactate or acetate, comprising contacting lignocellulosic biomass with a genetically modified thermophilic or mesophilic microorganism.

Detoxification of biomass derived acetate via metabolic conversion to ethanol, acetone, isopropanol, or ethyl acetate

Host cells, comprisingexpressing heterologous cellulases produce ethanol from cellulose In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose The recombinant yeast strains and co-cultures of the yeast strains can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of sacchanfication and fermentation processes.

The present invention describes a number of different microorganisms that have been genetically-engineered to optimize ethanol production. The present invention also describes methods of using such microorganisms to efficiently make ethanol.

Genetically-engineered ethanol-producing bacteria and methods of using

Growing community of inventors

Lebanon, NH, United States of America

Alan Benjamin Gilbert