Other

Detroit R & D, Inc.

Alan A Dombkowski

Hyesook Kim

Michael David Kane

Brian Brummeler Haab

Raymond F Novak

Aaron C Nagel

An antibody microarray screen including a substrate, monoclonal and polyclonal antibodies that are purified immunoglobins, wherein the antibodies are spotted on predetermined positions on the substrate, and fluids unprocessed for immunoglobulin isolation (e.g., anti-sera, ascites fluids, or hybridoma culture media), wherein the unprocessed fluids are spotted on the predetermined positions on the substrate. Production of drug-metabolizing enzyme antibody microarrays containing closely related cytochromes P450 is disclosed. Methods of manufacturing an antibody microarray, an internal control molecule for use in an antibody microarray, a method of determining optimal spotting concentrations of IgG and a method to increase a detectable signal with microarray analysis are disclosed.

Chip production, hybridization and data interpretation for antibody and protein microarrays

The invention comprises compositions and systems to identify and compare expressed genes in a given in vivo or in vitro RNA sample, as well as the relative difference in mRNA expression between two or more samples, where desired. Furthermore, the invention comprises compositions and systems to identify novel genes. The invention comprises, without limitation, one or more mRNA specific identimers for use in reverse transcription that themselves comprise an oligo-T nucleotide sequence (at the 5' end) linked to a nucleotide sequence VNx (at the 3&#x2032; end) where the V nucleotide immediately adjacent to the oligo-T segment is not a T.

Compositions and systems for identifying and comparing expressed genes (mRNAs) in eukaryotic organisms

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Canton, MI, United States of America

Alan A Dombkowski