Biomerieux, Inc.

Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Alain Troesch

Ali Laayoun

Jean-Claude Boulain

Corinne Jay

Laurent Mesta

Frédéric Ducancel

Bruno Lacroix

Janie Dassa

Bruno H Muller

The present invention pertains to a mutated T7 RNA polymerase and its use, the T7 RNA polymerase being mutated at position 744, the glutamine (Q) being replaced by an amino acid selected from arginine (Q744R), leucine (Q744L) or proline (Q744P).

Mutated T7 RNA Polymerases

A method of amplifying a nucleic acid of interest comprises (i) treating a biological sample chemically or enzymatically to permit conversion of one type of nucleic acid base to another type of base, (ii) purifying the treated biological sample before adding amplification primers and amplification reagents to the biological sample, (iii) adding the amplification primers and amplification reagents to the biological sample, each primer being constituted of three different types of bases and being specific to a converted nucleic acid of interest or to a nucleic acid that is complementary to the converted nucleic acid of interest, and (iv) amplifying the converted nucleic acid of interest provided that the nucleic acid of interest was present in the biological sample. In the method, amplification of contaminating nucleic acids is avoided by converting the one type of nucleic acid base to another type of base prior to adding the amplification reagents to the biological sample.

Methods and kits for avoiding amplification of contaminating nucleic acids

The present invention relates to a method for detecting and/or identifying bacteria of the genus, comprising the following steps:

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Genas, France

Alain Troesch