Rhone-poulenc Agrochimie

Bayer Cropscience S.a.

Aventis Cropscience S.a.

Bayer Sas

Bayer Cropscience Gmbh

Aventis Cropscience Gmbh

Alain Sailland

Georges L Freyssinet

Eric Paget

Michel Lebrun

Ken Pallett

Richard Derose

Anne Rolland

Bernard Leroux

Bernard Pelissier

Olivier Zink

Bernd Laber

Marco Busch

Jean-Marc Ferullo

Florence Dumas

Matthew Rodgers

Frédéric Schmitt

Philippe Boudec

H&eacute;l&egrave;ne Bourdon

Kerstin Fischer

Eric Degryse

Claude Gigot

Nicole Chaubet

Thomas Edward Vrabel

Michel Matringe

Frederic Schmitt

The present invention relates to a novel expression cassette comprising a nucleic acid sequence encoding an EPSPS. In particular, the present invention relates to a novel expression cassette comprising, in the direction of transcription, functionally linked to one another, a promoter regulatory sequence which is functional in plant cells or plants, a nucleic acid sequence encoding an EPSPS and a terminator sequence which is functional in plant cells or plants, characterized in that the promoter regulatory sequence is a nucleic acid sequence chosen from the promoter regulatory sequences of the CsVMV (Cassava Vein Mosaic Virus) plant virus.

Expression cassette encoding a 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) and herbicide-tolerant plants containing it

The invention concerns a novel method for making herbicide-tolerant plants, in particular to HPPD inhibiting herbicides, the nucleic acid sequences coding for enzymes capable of being used in said method, expression cassettes containing them and transgenic plants comprising at least one of said expression cassettes.

Herbicide-tolerant plants through bypassing metabolic pathway

1. Chimeric gene containing several herbicide tolerance genes, plant cell and plant which are tolerant to several herbicides. 2. The plant is tolerant to several herbicides at the same time, in particular to the inhibitors of HPPD and to those of EPSPS and/or to the dihalohydroxybenzonitriles. 3. Use for removing weeds from plants with several herbicides.

Chimeric gene with several herbicide tolerance genes, plant cell and plant resistant to several herbicides

DNA sequence of a gene of hydroxy-phenyl pyruvate dioxygenase and production of plants containing a gene of hydroxy-phenyl pyruvate dioxygenase and which are resistant to herbicides. DNA sequence of a gene of hydroxy-phenyl pyruvate dioxygenase; isolation from a bacteria or a plant; utilization for obtaining plants tolerant to herbicides.

Chimera gene with several herbicide resistant genes, plant cell and plant resistant to several herbicides

The invention concerns a method for enzymatic preparation of homogentisate (HMO) from 4-hydroxypyruvate (HPP), characterized in that it consists in carrying out in a suitable reaction medium the following enzymatic reactions: enzymatic conversion of HPP into 4-hydroxyphenylacetate (HPA) with a first appropriate enzyme; then an enzymatic conversion of HPA into HMO with a second appropriate enzyme.

Method for enzymatic preparation of homogentisate

The invention concerns a nucleic acid encoding a chimeric hydroxyphenyl pyruvate dehydrogenase (HPPD) enzyme formed from a nucleic acid encoding the N-terminal portion of a first plant HPPD in combination with nucleic acid encoding the C-terminal portion of a second plant HPPD, wherein the N-terminal portion and the C-terminal portion are separated by a linker peptide. The nucleic acid can be used to transfom a cell or an organism, such as a plant, to provide tolerance to herbicides. The invention also concerns methods of making and using plants transformed with the nucleic acid.

Chimeric hydroxyl-phenyl pyruvate dioxygenase, DNA sequence and method for obtaining plants containing such a gene, with herbicide tolerance

The present invention relates to DNA molecules of plant origin encoding a modified 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme wherein the a first EPSPS coding sequence that normally encodes a threonine residue of a mature EPSPS sequence is modified to encode isoleucine of the mature EPSPS sequence, and a second EPSPS coding sequence that normally encodes a proline residue of a mature EPSPS sequence is modified to encode serine of the mature EPSPS sequence, wherein the first and second residues are respectively located at relatively positions 102 and 106 of a mature EPSPS sequence encoded by said DNA molecule; and the production of a transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g., glyphosate. The mutated enzyme, which substantially maintains the catalytic activity of the wild-type enzyme, allows for increased tolerance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells.

Mutated 5-enolpyruvylshikimate-3-phosphate synthase, gene coding for said protein and transformed plants containing said gene

1) Chimeric gone for conferring to plants an increased tolerance to a herbicide. 2) It comprises, in the direction of transcription, a promoter region, a transit peptide region, a sequence encoding glyphosate tolerance and a polyadenylation signal region, wherein the promoter region consists of at least one promoter of a plant histone gene enabling the expression of the herbicide tolerance protein in the regions of glyphosate accumulation. 3) Production of glyphosate-tolerant plants.

Chimeric gene for the transformation of plants

An isolated gene from Pseudomonas is described which expresses a hydoxy phenyl pyruvate dioxygenase. Also described are chimeric genes for introduction into plants to overexpress a hydoxy phenyl pyruvate dioxygenase and produce plants which are tolerant to herbicides.

DNA sequence of a gene of hydroxy-phenyl pyruvate dioxygenase and production of plants containing a gene of hydroxy-phenyl pyruvate dioxygenase and which are tolerant to certain herbicides

Chimeric gene for conferring to plants an increased tolerance to a herbicide having as its target EPSPS comprises, in the direction of transcription, a promoter region, a transit peptide region, a coding sequence for glyphosate tolerance and a polyandenylation signal region, wherein the transit peptide region comprises, in the direction of translation, at least one transit peptide of a plant gene encoding a plastid-localized enzyme and then a second transit peptide of a plant gene encoding, a plastid-localized enzyme. Production of glyphosate-tolerant plants is disclosed.

The present invention relates to a nucleic acid sequence encoding a mutated hydroxyphenylpyruvatedioxygenase (HPPD) which exhibits improved tolerance to HPPD inhibitors, to a chimeric gene which comprises this sequence as the coding sequence, and to its use for isolating or obtaining plants which are resistant to certain herbicides.

Mutated hydroxyphenylpyruvate dioxygenase, DNA sequence and isolation of plants which contain such a gene and which are tolerant to herbicides

The present invention provides a DNA sequence encoding an oxalate oxidase. The oxalate oxidase may be used for the resistance of plants to diseases caused by Sclerotinia sp. It may be provided by a chimeric gene and a vector containing the coding sequence. It may be used to confer on plants an increased resistance to diseases caused by Sclerotinia sp.

Growing community of inventors

Lyons, France

Alain Sailland