Biomerieux, Inc.

UniversitÉde Strasbourg

Cnrs

Université De Caen Normandie

Biomerieux Sa

Alain Laurent

Ali Laayoun

Sylvain Ursuegui

Arnaud Burr

Frédéric Fabis

Bernard F Mandrand

Carole Chaix

Mitsuharu Kotera

Laurent Mesta

Eloy Bernal-Mendez

Daniela Zsoldos

Nicolas Spinelli

Raphäel Veyret

Thomas Cailly

Mitsuhara Kotera

A method for extracting nucleic acids using suitable solid supports, for example silica-based. In particular, the method includes a step of capturing nucleic acids by placing the sample in contact with a suitable solid support, characterized in that, prior to the capture step, said method includes a step of treating the sample with at least one reagent for masking the amine or carboxylic acid functional groups of the proteins and/or polysaccharides of the sample.

Method for extracting nucleic acids

The present invention concerns reagents for the reversible protection of biological molecules. It relates in particular to compounds derived from azaisatoic anhydride and their uses for the protection of biological molecules, particularly enzymes, in order to block their activity. The invention also relates to the biological molecules protected in this manner and to the methods for making use of these reagents.

Reagents for reversibly protecting biological molecules

A three-layer complex including at least one magnetic compound, at least one inorganic silicate and at least one compound having an affinity for the at least one magnetic compound and/or the at least one inorganic silicate. The three-layer complex, and associated methods, may be applicable in the field of in vitro diagnostics.

Multilayer complex, method for manufacturing said complex and use of said complex

The present invention relates to a method of functionalization of at least one ribonucleic acid (RNA) molecule, contained in a liquid sample, which includes the following steps: a) providing at least: one binding molecule consisting of an aza-isatoic anhydride or a derivative thereof, one group of interest, and one linkage joining the binding molecule to the group of interest, b) reacting the anhydride function of the binding molecule with at least one hydroxyl group carried: in position 2' of the ribose of one of the RNA nucleotides, and/or in position(s) 2&#x2032; and/or 3&#x2032; of the ribose of the nucleotide at the terminal 3&#x2032; end of the RNA, and obtaining an aza-anthranilate that joins, by means of the linkage, the RNA to the group of interest.

Methods of functionalization and reagents used in such methods using an aza-isatoic anhydride or a derivative thereof, biological molecules thus treated and kits

A method of transcriptional amplification includes: (a) obtaining a mixture by combining (i) a biological sample comprising nucleic acids, (ii) amplification primers, (iii) amplification reagents including enzymes required for amplification, and (iv) at least one polyol capable of stabilizing the enzymes required for amplification; (b) denaturing the nucleic acids by heating the mixture at a temperature above 41&#xb0; C.; and (c) transcriptionally amplifying at least one target nucleic acid at a temperature above 41&#xb0; C. when present in the mixture.

Method for transcriptional amplification of nucleic acids combining steps of different temperatures

A process for synthesis of a labelling reagent, a process for the labelling of a biological molecule, a labelled biological molecule obtained by the process, a process for labelling and fragmentation of a single or double strand nucleic acid, a labelled nucleic acid capable of being obtained by the process, a kit for detection of a target nucleic acid containing a labelled nucleic acid, a solid support onto which is attached a reagent and a process for capture of nucleic acids.

Labelling reagents having a pyridine nucleus bearing a diazomethyl function, process for synthesis of such reagents and processes for detection of biological molecules

The present invention relates to a labeling reagent of formula: in which:

Marking reagents bearing diazo and nitro functions, methods for the synthesis of such reagents and methods for detecting biological molecules

The present invention relates to a labeled oligonucleotide comprising a first nucleotide segment and a second nucleotide segment, complementary to a target sequence, a fluorophore, a quencher and at least one alpha-anomeric nucleoside. The invention also relates to the use of such an oligonucleotide and also to a process using such an oligonucleotide.

Labeled oligonucleotide

The invention relates to novel pyrrole derivatives of the formula (I) which make it possible to immobilize and address oligonucleotides by electropolymerization. Said invention also relates to thus produced electroactive polymers and to methods for using them for detecting, identifying and dosing analytes in a sample. (I) wherein Ris one type of oligonucleotide, Y is S or O, X is a spacer arm selected from &#x2014;(CH)&#x2014;O&#x2014;, &#x2014;(CH)&#x3c;SB&#x3e;P&#x3c;/SB&#x3e;O&#x2014;[(CH)&#x2014;O]&#x2014;, &#x2014;(CH2); &#x2014;CO&#x2014;NR'&#x2014;(CH)&#x2014;O&#x2014;, &#x2014;CH&#x2014;NCH&#x2014;(CH)&#x2014;O&#x2014;, &#x2014;CH)CO&#x2014;NR&#x2032;&#x2014;[CH)&#x2014;O]&#x2014;, &#x2014;(CH)NCH[(CH)&#x2014;O]&#x3c;SB&#x3e;S&#x3c;/SB&#x3e;&#x2014;, R&#x2032; is H or CH, n is an integer number ranging from 1 to 5, p is an integer number ranging from 1 to 2, q is an integer number ranging from 1 to 4, r is an integer number ranging from 1 to 3, r&#x2032; is an integer number ranging from 1 to 3, s is an integer number ranging from 1 to 3, n, p, q, r, r&#x2032; and s are identical or different, a pyrrole cycle is substituted in a position 2, 3, 4 or 5.

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Grenoble, France

Alain Laurent