Institut Francais Du Pe'trole

Other

Université De Nantes

Centre National De La Recherche Scientifique

Ifp Energies Nouvelles

Centre National De La Recherche Scientifique-cnrs

Couillard, Francois

Alain Foucault

Daniel Durand

Jack Legrand

François Couillard

Patrick Durand

Luc Marchal

Francois Couillard

Jérémy Pruvost

Roland De La Poype

Fran&ccedil;ois De La Poype

G&eacute;rard Patissier

Jean-Michel Rosant

Jeremy Pruvost

A method is provided for extracting cellular compounds from micro-organisms. The method includes culturing the micro-organisms and extracting the cellular compounds from the micro-organisms. Each step is carried out in a continuous manner, wherein the extraction step is carried out separately from the culture step and in conditions of biocompatibility with the micro-organisms. The method further includes at least one step of recovering the cellular compounds and at least one step of recirculating the micro-organisms towards the culture step.

Method for the intensive extraction of cellular compounds from micro-organisms by continuous culture and extraction, and corresponding device

The invention relates to a method for separating constituents of a liquid feed by liquid-liquid centrifugal chromatography and to a device for implementing it. Constituents (A, B) having different partition coefficients such that they are respectively carried along at unequal velocities by a light solvent (l) and a heavier solvent (L), the feed is continuously injected at an intermediate point of a separation column (col), successive cycles comprising alternately a heavy solvent injection stage and a light solvent injection stage are carried out respectively at the two ends of the column consisting of the interconnection in series of at least one set of separation cells, and the separated solvents (FA, FB) are collected during each one of said stages at the column ends opposite the solvent injection points.

Method and device for separating constituents of a liquid feed by liquid-liquid centrifugal chromatography

Method for sizing the cells of a centrifugal liquid-liquid chromatography 'column' (CPC column) consisting of stacked discs on which the cells connected in cascade (in series) by small channels are engraved. Rotation of the stack creates a high centrifugal acceleration field which makes it possible to keep a liquid phase referred to as stationary phase motionless, whereas a mobile phase circulates along the CPC column. The cells are three-dimensional, with two dimensions (L, l) of the cells oriented in a plane substantially normal to the axis of rotation (&#x3a9;) of the disc and a third dimension (e) oriented in a direction substantially parallel to the axis of rotation, and selected so as to be at least equal to one of the other two dimensions (L, l), thus providing higher efficiency. When the scale of the devices has to be changed, the size of the cells is modified ensuring that, in any case, this third dimension (e) is favored so as to be as great as possible. Applications: design of analytic or preparative chromatography devices.

Method for optimally sizing cells of a centrifugal partition chromatography device

The invention relates to a method for separating constituents of a liquid feed by liquid-liquid centrifugal chromatography and to a device for implementing it. Constituents (A, B) having different partition coefficients such that they are respectively carried along at unequal velocities by a light solvent () and a heavier solvent (L), the feed is continuously injected at an intermediate point of a separation column (col), successive cycles comprising alternately a heavy solvent injection stage and a light solvent injection stage are carried out respectively at the two ends of the column consisting of the interconnection in series of at least one set of separation cells, and the separated solvents (FA, FB) are collected during each one of said stages at the column ends opposite the solvent injection points.

Method and device for separating constituents of a liquid charge by means of liquid-liquid centrifuge chromatography

A centrifuge partition chromatography device for separating a liquid into at least two phases, comprising at least one flat ring ( ) that can be rotationally driven around its axis, disposed in a substantially vertical position. Said ring ( ) comprises a plurality of cells ( ) whose dimensions are greater in a first direction than the dimensions in a second direction which is substantially perpendicular to the first, disposed side by side and distributed along the periphery of said ring ( ) in addition to being connected to each other in a series by inlet and outlet ducts ( ) terminating in the opposite respective ends of each cells ( ) enabling the circulation of liquid. The invention is characterized in that said ducts ( ) are disposed in such a way that the liquid supply jet penetrates the cells ( ) in a direction that includes at least one radial component relative to the axis of rotation of said ring ( ).

Cell centrifuge partition chromatography device

Growing community of inventors

Saint-Nazaire, France

Alain Foucault