Aventis Pharma S.a.

Rhone-poulenc Rorer S.a.

Alain Famechon

Francis Blanche

Denis Thibaut

Jo&euml;l Crouzet

Nathalie Bamas-Jacques

Jean-Claude Barriere

Jean-Marc Paris

Gilles Dutruc-Rosset

Laurent Debussche

V&eacute;ronique Blanc

Lucia Ferrero

Beatrice Cameron

Joel Crouzet

B&eacute;atrice Cameron

Véronique Blanc

The present invention relates to novel variants of the PapM polypeptide of bacteria of thegenus which possess a substrate selectivity and/or an improved effectiveness compared to the wild-type polypeptide. It also relates to the nucleic acids encoding these variants, to the microorganisms which incorporate these nucleic acids and to their use for producing B components of Streptogramins.

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Novel group B streptogramin-like compounds of general formula (I), and a method for preparing streptogramines by mutasynthesis using a mutated micro-organism to influence the biosynthesis of at least one of the precursors of group B streptogramins are disclosed. Novel nucleotide sequences involved in the biosynthesis of said precursors, and their uses, are also disclosed.

Streptogramins and method for preparing same by mutasynthesis

A novel topoisomerase IV, nucleotide sequences coding for said enzyme, corresponding vectors, and the use of said enzyme for screening biologically active materials.

Topoisomerase IV, corresponding nucleotide sequences and uses thereof

The invention provides a method for preparing streptogramins using genetically-modified microorganisms to influence the biosynthesis of at least one of the precursors of the group B streptogramins. Cultures of the genetically-modified microorganisms are supplemented with a least one precursor that is different from the streptogramin precursor whose biosynthesis is altered and the streptogramins produced are recovered.

Streptogramins for preparing same by mutasynthesis

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Janville-sur-Juine, France

Alain Famechon