Takara Bio Usa, Inc.

Alain-Albert Mir

Jude Menlo Dunne

Maithreyan Srinivasan

Thomas David Schaal

Chun-Wah Lin

Shanavaz Loharasp D Nasarabadi

Patricio Espinoza

Patricio A Espinoza-Vallejos

The present disclosure provides methods, device, assemblies, and systems for dispensing and visualizing single cells. For example, provided herein are systems and methods for dispensing a dispense volume into a plurality of wells of a multi-well device, where, on average, a pre-determined number of cells (e.g., 1-20) are present in the dispense volume, and determining, via a cellular label, the number of cells present in each of the plurality of wells. Such dispensing and cell detection may be repeated a number of times with respect to wells identified as having less than the pre-determined number of cells in order increase the number wells in the multi-well device containing the desired number (e.g., a single cell).

Method for rapid accurate dispensing, visualization and analysis of single cells

Provided herein are systems and methods for whole genome amplification and sequencing. In particular, provided herein are systems and methods for detection of nucleic acid variants (e.g., rare variants) in limited samples.

Systems and methods for whole genome amplification

Growing community of inventors

Redwood City, CA, United States of America

Alain-Albert Mir