Keio University

National University Corporation National Higher Education and Research System

Akiyoshi Hirayama

Tomoyoshi Soga

Shoichi Maruyama

Hiroshi Abe

Shinichi Akiyama

An object of the present invention is to provide a method capable of detecting whether a subject has systemic lupus erythematosus (preferably nephrotic syndrome) with high accuracy without performing a renal biopsy, a biomarker for carrying out such detection, and the like. The concentration of 3',4′-didehydro-3′-deoxycytidine in urine collected from a test subject is measured, and this concentration is compared with a reference concentration for control, which allows to detect whether the above-mentioned test subject has systemic lupus erythematosus (preferably lupus nephritis) with high accuracy.

Method for detecting SLE

Processing a capillary distal end into acute-angle, an electrophoretic liquid passing hole through which electrophoretic liquid pass is opened in a flexible insulating plate. An electrodialysis-membrane is bonded covering the electrophoretic liquid passing hole; the capillary is securely bonded to the insulating plate portion with no gap therebetween, the portion excluding electrophoretic liquid passing hole on the electrodialysis top-membrane. A crack forms at the capillary portion at the electrophoretic liquid passing hole, with the capillary entirely secured to the insulating plate except portion at the electrophoretic liquid passing hole. The capillary is securely bonded to the insulating plate; reservoir stores electrophoretic liquid on the insulating plate-side to which the capillary is unsecured. An electrode insertion hole into which an electrode is inserted opened in the reservoir upper portion; the electrode is securely inserted into the electrode insertion hole. By this, high-sensitivity measurement is using a spray device for sheathless CE-MS.

Growing community of inventors

Tsuruoka, Japan

Akiyoshi Hirayama