Fuso Pharmaceutical Industries, Ltd.

Akira Okui

Nozomi Yamaguchi

Katsuya Kominami

Shinichi Mitsui

Hidetoshi Uemura

Shinichi 202 Kitashirakawa-Koporasu Mitsui

Nozomi 285-79 Shingoryoguchi-Cho Yamaguchi

There are provided a polynucleotide encoding human BSSP6 serine protease and a vector and transformed host cells containing this human BSSP6 serine protease encoding polynucleotide.

Serine protease BSSP6

There are provided proteins having the amino acid sequences represented by SEQ ID NOS: 2, 4, 6, 8, 10, 12, 14, 16, 18 and 20; proteins having amino acid sequences derived from these amino acid sequences by deletion, substitution or addition of one to several amino acids; and nucleotide sequences encoding the same; transgenic non-human animals with altered expression level of a serine protease BSSP4; an antibody against BSSP4; and a method for detecting BSSP4 in a specimen by using the antibody.

Serine protease BSSP4

A protein expression vector containing (a) a nucleotide sequence encoding an IgG(&#x3ba;) or a trypsin secretory signal peptide, (b) a nucleotide sequence encoding a polyhistidine amino acid sequence, (c) a nucleotide sequence encoding an amino acid sequence comprising amino acid residues 36&#x2013;40 of SEQ ID NO:19 (Asp-Asp-Asp-Asp-Lys), which is cleavable by an enterokinase, and (d) a cloning site into which a nucleotide sequence encoding a target protein can be inserted, wherein (a), (b), (c) and (d) are assembled within the vector in the order recited.

Protein expression vector and utilization thereof

There are provided proteins having the amino acid sequences represented by SEQ ID NOS: 2 and 4; proteins having amino acid sequences derived from these amino acid sequences by deletion, substitution or addition of one to several amino acids; and nucleotide sequences encoding the same; transgenic non-human animals with altered expression level of a serine protease BSSP5; an antibody against BSSP5; and a method for detecting BSSP5 in a specimen by using the antibody. The BSSP5 provied by the present invention can be used for treating and diagnosing various diseases such as Alzheimer's disease (AD), epilepsy, cancer, inflammation, sterility and prostatic hypertropy and detecting pancreatitis in various tissues including brain, prostate gland, placenta, pancreas and spleen.

Serine protease BSSP5

There are provided a human BSSP6 serine protease and a pharmaceutical composition containing this serine protease. Also provided is an antibody to this serine protease or to a fragment thereof.

There are provided proteins having the amino acid sequences represented by SEQ ID NOS: 2, 4, 6, 8 and 10; proteins having amino acid sequences derived from these amino acid sequences by deletion, substitution or addition of one to several amino acids; and nucleotide sequences encoding the same; transgenic non-human animals with altered expression level of a serine protease BSSP2; an antibody against BSSP2; and a method for detecting BSSP2 in a specimen by using the antibody.

Serine protease BSSP2

A monoclonal antibody binding selectively to neurosin obtained from hybridomas, in particular, strain 2B2-6 and strain S2E5 showing stable proliferation ability. These hydridomas are obtained by fusing mouse spleen cells having a high antibody titer against neurosin with mouse-derived myeloma cells, screening fused cells being highly reactive with neurosin, and thus producing an antibody binding specifically to neurosin. By using this antibody, various diseases in which neurosin participates can be diagnosed.

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Nara, Japan

Akira Okui