Toyo Jozo Kabushiki Kaisha

Kokusan Chemical Works Limited

Akira Ohtsuka

Susumu Watanabe

Kunio Matsumoto

Tsutomu Hirata

Yoshitaka Kagimoto

Kiyoharu Takahashi

A method for assay of enzyme activity which comprises treating an amide compound of the formula ##STR1## with peptidase; treating thus-liberated amine of the formula ##STR2## with an oxidase which consume oxygen and forms pigment by oxidative condensation of said amine with a coupler of the formula ##STR3## and quantitatively measuring the detectable changes. Also disclosed is an assay method which comprises treating an amide compound of the formula ##STR4## or a salt thereof, with peptidase to liberate an aniline derivative of the formula ##STR5## oxidizing said aniline derivative; and quantitatively measuring detectable change.

Assaying peptidase enzyme activity

A compound of the formula ##STR1## wherein R is L-leucyl or .gamma.-L-glutamyl and X and Y are halogen and are the same or different, or a salt thereof. These compounds are useful as synthetic substrates for assaying peptidase activity.

Novel synthetic substrate and assay method using the same

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Kanagawa, Japan

Akira Ohtsuka