Takara Shuzo Co., Inc.

Nippon Shinyaku Company, Ltd.

Akira Obayashi

Susumu Matsui

Nobutsugu Hiraoka

Tsutomu Taniguchi

Osamu Tanabe

Teruya Nakamura

Keiko Kita

Hiroshi Nakajima

Ikunoshin Kato

Takeshi Sakai

Shingo Matsumura

Kazuo Nakajima

Hirokazu Kotani

Yukita Kawano

Katsuhiko Kusakabe

Hiroyuki Uchida

Hitoshi Maruyama

Yoshiaki Hirata

Hamao Umezawa

Kazutoh Takesako

Teruhiko Beppu

Masaji Ohno

Masakuni Ozaki

Akihiro Kondo

Takuma Sasaki

Sumiko Akiyoshi

Yoshio Yoshihama

Hiroyuki Kuroda

Sadaji Yokoyama

Osamu Suzuka

Masao Murayama

Hisao Watanabe

Yoshie Kimizuka

Sumihiro Hase

Tomoko Shiomi

Hiroko Yokota

Toshinori Miyabe

Kazuki Yamamoto

Yukuo Ishizaki

Mitsuo Kasai

Atsushi Ohshima

Asuka Kagaya

SP6 bacteriophage RNA polymerase is produced by cultivating a new microorganism (particularly new strains of Escherichia coli) harboring a plasmid that carries SP6 bacteriophage RNA polymerase gene and recovering SP6 bacteriophage RNA polymerase from the culture broth. SP6 bacteriophage RNA polymerase gene is provided as are new microorganisms harboring a plasmid that carries SP6 bacteriophage RNA polymerase gene.

RNA polymerase gene, microorganism having said gene and the production

Method of cultivating mushrooms in a culture medium containing aluminum or a particular aluminum compound.

Method of cultivating mushrooms

A method for the fluorescent labelling of a sugar or a sugar chain by labelling the reducing end of the sugar or sugar chain with a fluorescent substance, wherein the improvement comprises first forming a Schiff base by reacting the surgar or sugar chain with the fluorescent substance in the presence of an acid which is substantially free from water, and second, reducing said Schiff base.

Method for the fluorescent labelling of sugars

NAD(P)H oxidase is disclosed having the following enzymological properties:

Novel NAD(P)H oxidase

The invention provides novel strains of Basidiomycetes and more particularly, novel strains of Lyophyllum ulmarium. The novel strains are characterized by having a cap which is not concave when the fruiting body has matured. A process is provided comprising inoculation of Lyophyllum ulmarium with a cap which is not concave when the fruiting body has matured on a medium to form the fruiting body. Also provided is a method comprising the mating of Lyophyllum ulmarium Lu 1-8 with other Lyophyllum ulmarium and the harvesting of Lyophyllum ulmarium with a cap which is not concave when the fruiting body has matured in a term of cultivation up to harvest of the fruiting body that does not exceed 100 days.

Novel strains and their cultivation

A method is provided for the detection of a disease associated with the metabolic abnormality of L-fucose in a subject. The concentration of free L-fucose in a specimen from the subject is determined and the determined concentration is compared with a normal concentration of free L-fucose.

Method for the detection of diseases associated with the metabolic

A method is provided for the quantitative determination of L-fucose in a sample solution. According to the method an L-fucose dehydrogenase having its optimum pH around neutrality is allowed to act upon the sample solution in the presence or absence of an .alpha.-L-fucosidase, and the amount of reduced nicotinamide adenine dinucleotide thus formed is measured.

Quantitative determination of L-fucose

An enzyme composition comprising a series of enzymes used for DNA base sequence analysis by gel electrophoresis and a dihydric alcohol.

Enzyme composition

The invention provides restriction endonuclease Mf1 I capable of recognizing the base sequence as shown below on a double-stranded DNA molecule and cleaving the DNA chain at the arrow-marked positions, but has no such action when A is methylated

New restriction enzyme and process for producing the same

A process for producing a restriction endonuclease capable of recognizing the nucleotide sequence ##STR1## (wherein A, C, G and T represent adenosine, cytidine, guanosine and thymidine, respectively) on a DNA chain and specifically cleaving the double-stranded chain at the arrow-marked positions. This process comprises growing a microorganism belonging to the genus Halococcus and capable of producing said restriction endonuclease, and collecting the enzyme thus formed from the culture broth.

Process for producing restriction enzyme

The feature of the present invention is a process for preparing compounds represented by general formula (Ia) described below: ##STR1## wherein X.sub.1, X.sub.2, X.sub.3, R.sub.1, R.sub.2, R.sub.3 and R.sub.7 are as defined in formula (I) and R.sub.10 represents a lower alkyl group, or acid salts thereof, which process is characterized by using the guanidylfungins (II) as raw materials, reacting these guanidylfungins with alcohols (III) in the presence of an acid catalyst, and then hydrolyzing the malonic acid monoester (IV).

Guanidylfungin derivatives and their production

A restriction endonuclease having the ability to recognize the same base sequence and cleavage sites as SacII and SstII can be produced from Gluconobacter and isolated in pure form because no other restriction enzyme is formed.

Process for producing a restriction enzyme

The invention relates to a high molecular weight .beta.-1,3-glucan composed of repeated glucopyranose units, each unit being represented by the formula: ##STR1## The .beta.-1,3-glucan exhibits anticarcinogenic activity. A process for producing said glucan is also claimed.

Polysaccharides having anticarcinogenic activity and method for

The present invention provides a process for preparing a optically active .beta.-(S)-aminoglutaric acid monoalkyl ester of the formula: ##STR1## wherein R is a lower alkyl group having 1 to 4 carbon atoms, which comprises subjecting a .beta.-N-protected aminoglutaric acid dialkyl ester of the formula: ##STR2## wherein each R is as defined above and A is an easily removable protecting group, to an action of an enzyme produced by a microorganism belonging to the genera Flavobacterium, Achromobacter, Xanthomonas, Alcaligenes, Serratia, Gluconobacter, Chromobacterium and Acetobacter, to selectively hydrolyze only one of the two ester groups, and then removing the protecting group.

Method of preparing optically active .beta.-(S)-aminoglutaric acid

A polysaccharide having anticarcinogenic activity, and a process for its production from a strain of the genus Isaria of the class Hyphomycetes.

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Uji, Japan

Akira Obayashi