Agency of Industrial Science and Technology

Akira Kamibayashi

Hideo Suzuki

Yoshitsugu Kosugi

Aizo Yamauchi

Hidekatsu Maeda

Atsushi Mori

Harumi Yoshida

Yoshiko Ozawa

Munetaka Sato

Makoto Endo

A lipid hydrolyzing enzyme-containing substance is brought into contact with an insoluble carrier having a hydrophobic organic compound attached thereto in the form of a hydrophobic residue so that the lipid hydrolyzing enzyme is adsorbed by the hydrophobic residue attached to the insoluble carrier. Then, the lipid hydrolyzing enzyme adsorbed on the carrier is isolated by elution.

Method for adsorption and elution of lipid hydrolyzing enzymes

In a method for producing sucrose from beets or beet molasses by the deionization process using ion-exchange resins or the saccharate process or in a method for producing sucrose from beets without resorting to either of the two processes mentioned above, .alpha.-galactosidase is allowed to act upon the sugar solution, while in process, so as to hydrolyze the raffinose contained therein into sucrose and galactose. The raffinose-hydrolyzed sugar solution is returned to the process following the stage at which the sugar solution is withdrawn. Thereafter, the sugar solution is treated in the fixed sequence to effect the recovery of sucrose contained in the sugar solution.

Method for increasing yield of sucrose

Immobilized enzymes are produced by adding radioresistant enzymes to 5-25% aqueous solutions of polyvinyl type high molecular compounds and irradiating the enzyme-containing solutions with a high dosage of ionizing radiant rays in an inert atomsphere or under a decreased pressure for thereby gelling said solutions.

Method for production of immobilized enzyme

Growing community of inventors

Chiba, Japan

Akira Kamibayashi