Chugai Seiyaku Kabushiki Kaisha

Akira Hayasaka

Tomoyuki Igawa

Atsuhiko Maeda

Tsutomu Arakawa

Shojiro Kadono

Yuji Hori

Shun Shimizu

Mika Sakurai

Tadao Yamazaki

Taro Miyazaki

Hiroki Kawauchi

Yasuo Sekimori

Kanako Tatsumi

Masami Hasegawa

Masaki Kamimura

Yoshinori Narita

Masaya Kakuta

Masakazu Fukuda

Naoka Hironiwa

Takeaki Kawai

Yoshiki Hayashi

Machiko Fujino

Takayuki Kamikawa

Nasa Savory

Futa Mimoto

An objective of the present disclosure is to provide anti-CD137 antigen-binding molecules which have immunocyte-activating effect, cytotoxic activity, or anti-tumor activity, and meanwhile have reduced effect on non-tumor tissues such as normal tissues and produce less side effects, and methods of using the same. Anti-CD137 antigen-binding molecules which have immunocyte-activating effect, cytotoxic activity, or anti-tumor activity, and meanwhile have reduced effect on non-tumor tissues such as normal tissues and produce less side effects, are provided by discovering and producing CD137 antigen-binding molecules whose binding activity to CD137 depends on various substances (for example, small molecule compounds) in target tissues. Methods of using the same, pharmaceutical formulations, and such are also provided. The present disclosure also provides an antigen-binding molecule whose binding activity to an antigen varies depending on a small molecule compound, a preparation method thereof, and uses thereof.

Anti-CD137 antigen-binding molecule and utilization thereof

The inventors discovered that viscosity of a protein solution can be estimated by measuring the apparent particle size or apparent molecular weight by a small angle X-ray scattering (SAXS) method or X-ray solution scattering method, which enables measurement of small amounts of samples, and then correlating those measurement results with viscosity of the protein solution.

Method for measuring viscosity of protein solution

The present invention provides stabilized preparations containing an antibody in a glycine buffer and/or a histidine buffer and also provides processes for preparing a protein-containing stabilized preparation, comprising adjusting the pH with a basic amino acid or a basic amino acid derivative or a salt thereof.

Stabilized anti-interleukin-6 antibody-containing preparations

The present inventors examined use of citric acid buffers for suppressing cryoprecipitation of IgM at a pH range and salt concentration suitable for storing IgM. As a result, the present inventors discovered that citric acid buffers significantly suppress cryoprecipitation.

Methods for stabilizing protein solutions

Growing community of inventors

Shizuoka, Japan

Akira Hayasaka