Suntory Holdings Limited

Kanzaki Paper Manufacturing Co., Ltd.

Akio Noguchi

Harukazu Fukami

Misa Ochiai

Masahiro Nakao

Yukio Takayama

Mitsuru Kondo

Yuko Fukui

Eiichiro Ono

Masako Mizutani

Nobuo Kanda

Takao Matsushita

The present invention provides a method for preparing a glycoside of a flavonoid compound, which comprises the step of treating flavonoid and a glycosyl donor with an enzymatic agent having glycosylation activity and being derived from the genus(preferablyor). Such a flavonoid compound includes a catechin compound or a methylated derivative thereof, and the glycosyl donor includes a carbohydrate containing a maltotriose residue (preferably maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, dextrin, &#x3b3;-cyclodextrin or soluble starch). Glycosides obtained by the present invention have higher water solubility, improved taste, and increased stability. The present invention also provides novel glycosides of catechin compounds, which are obtained by the method of the present invention.

Method for glycosylation of flavonoid compounds

The present invention provides a novel UDP-glucuronosyltransferase and a polynucleotide encoding the same, for example, a polynucleotide comprising nucleotides 1 to 1359 of SEQ ID NO: 4, nucleotides 1 to 1365 of SEQ ID NO: 10, nucleotides 1 to 1371 of SEQ ID NO: 12, and nucleotides 1 to 1371 of SEQ ID NO: 22; or a polynucleotide encoding a protein having the amino acid sequence of SEQ ID NOS: 5, 11, 13 or 23. The invention provides a novel UDP-glucuronosyltransferase with a broad substrate specificity.

Polynucleotide encoding UDP-glucuronyl transferase

The present invention provides novel glycosyltransferase proteins produced by ascomycetous filamentous fungi (preferably species belonging to the genus, more preferably), as well as genes encoding the same. Among novel enzyme proteins provided by the present invention, particularly preferred is an enzyme protein obtained from the culture supernatant ofstrain IAM5141. The novel enzymes of the present invention allow glycosylation of flavonoid compounds to thereby improve their water solubility.

Polypeptide having glycosylation activity on a flavonoid

Polynucleotide encoding a polypeptide having glycosylation activity on a flavonoid

A record material utilizing the color forming reaction between a substantially colorless chromogenic material and an electron accepting reactant material is disclosed. The colorless chromogenic material used comprises a mixture of the first component consisting of at least one specified phthalide compound having two vinyl linkages and the second component of another kind of chromogenic material which is different from said first component. The first component of the chromogenic material is used in an amount of 5 to 80%, preferably 7 to 50%, by weight with respect to the amount of the second component and in such an amount that the reflectance at any wavelength within the range of 580 nm to 880 nm, preferably within the range of 500 to 880 nm of color images developed by said color forming reaction is less by at least 55% than that of said record material before color developing.

Record material

In a heat-sensitive recording material in which a resin layer is formed on a recording layer containing at least a color forming material and a color developing material which forms a color when reacted with the color forming material by contact, a heat-sensitive recording material which is characterized in that the resin is essentially composed of an acetoacetylated polyvinyl alcohol and/or a copolymer of vinyl alcohol, acrylonitrile and optionally an other copolymerizable monomer.

Growing community of inventors

Osaka, Japan

Akio Noguchi