Jcr Pharmaceuticals Co., Ltd.

Akio Matsuo

Hajime Hiratani

Junichi Koga

Hiroyuki Shirono

Antiserum which can recognize all subtypes of human leukocyte interferon is prepared from the blood of an animal immunized with partially purified human leukocyte interferon obtained from a culture of human leukocyte stimulated with Sendai virus. The antiserum is added to a column whereon concentrated culture broth of human leukocyte has been immobilized to adsorb impurities, the effluent is added to a column whereon partially purified human leukocyte interferon has been immobilized to adsorb anti-human leukocyte interferon antibody and then the antibody is eluted from the column. The antibody thus obtained recognizes all subtypes of human leukocyte interferon and can be separated by a chromatography to each monoclonal antibody which recognizes a single subtype. Employing these antibodies, the subtypes of human leukocyte interferon or their antibodies in a sample can be analized or assayed.

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Kobe, Japan

Akio Matsuo