Hiroshima University

Kawasaki Steel Corporation

Other

Primetals Technologies Japan, Ltd.

Japan Science and Technology Agency

Mitsubishi Jukogyo Kabushiki Kaisha

Mitsubishi Jukogyo Kabushiki Kaisha Hiroshima Machinery Works

Kawasaki Steel Corporation Chiba Works

Kawasaki Steel Corporation Et Al.

Terumo Kabushiki Kaisha

Akio Kuroda

Hideyuki Nikaido

Toshiaki Amagasa

Shigeru Isoyama

Takeshi Hirabayashi

Hirosuke Yamada

Kunio Miyamoto

Toshisada Takechi

Yoshiki Mito

Kanji Hayashi

Nozomu Tamura

Kazuo Morimoto

Kazuya Tsurusaki

Ikuo Wakamoto

Hiroyuki Nakano

Norio Takashima

Takahiro Yamasaki

Junzo Nitta

Shuji Amanuma

Hideo Sakamoto

Michio Hashimoto

Toshinobu Eguchi

Fumihiro Maeda

Masashi Osada

Ryuichi Hirota

Kenji Horii

Shinya Kanemori

Akira Sako

Kazunori Nagai

Katsuhiro Takebayashi

Hiroya Sato

Tatsunori Sugimoto

Takenori Ishida

Kiyoshi Izumi

Kenichi Noda

Takeshi Ikeda

Kazutaka Nomura

Kei Motomura

Tomoki Nishimura

Hisakage Funabashi

Angles of an upper-side pair of an upper work rollA and an upper backup rollA, and a lower-side pair of a lower work rollB and a lower backup rollB are adjusted in a state where the upper-side pair is kept parallel and in a state where the lower-side pair is kept parallel. Thereafter, work-roll pressing apparatusesA andB, work-roll position control apparatusesA andB, backup-roll pressing apparatusesA andB, and backup-roll position control apparatusesA andB are controlled such that the angles of the upper work rollA and the lower work rollB are adjusted in a state where the angles of the upper backup rollA and the lower backup rollB are maintained.

Hot rolling mills and hot rolling methods

A hot rolling millincludes a control apparatusthat adjusts an angle formed between an upper-side pair of an upper backup rollA and an upper work rollA and a lower-side pair of a lower backup rollB and a lower work rollB in a state where the upper-side pair is kept parallel and in a state where the lower-side pair is kept parallel. The hot rolling millis configured such that the work rollsA andB satisfy the condition that DW/Lb is equal to or smaller than 0.30 where DW is a diameter of the work rollsA andB, and Lb is the maximum strip width of a rolled material, and the hot rolling millperforms rolling in a state where a bending force is applied to the work rolls.

A control deviceobtains second pressing forces acting on work-side and drive-side roll chocksA andB on the basis of entry side and exit side first pressing forces, and controls at least one of work roll position control devicesB andB and at least one of work roll pressing devicesB andB changing a position of at least one of the roll chocksA andB in such a manner that a difference between the work-side second pressing force and the drive-side second pressing force is equal to or smaller than a predetermined value.

Rolling mills and rolling methods

It is an object of an aspect of the present invention to provide (i) transformants, whose proliferation depends on phosphite, of various species of organism and (ii) a method for detecting the presence of a reduced phosphorous compound with use of such a transformant. Use is made of a transformant which is defective in functions of a gene encoding a phosphate transporter protein and a gene encoding a phosphate ester transporter protein and into which a gene encoding a hypophosphite transporter protein is introduced, a signal peptide of a hypophosphite binding protein being substituted with a signal peptide derived from a host or a species of organism closely related to the host.

Transformant, and method using said transformant to detect presence or absence of reduced phosphorous compound

The present invention provides a method for simply isolating exosomes from a sample containing exosomes. The present invention includes a complex forming step of forming a complex by binding an exosome in a sample to a particular peptide that contains lysines and is supported on a carrier and a dissociating step of dissociating the exosome from the complex by bringing the complex into contact with a dissociation buffer containing metal cations.

Method for isolating exosome and exosome isolation kit

In order to provide a transformant by which a high containment effect is obtained and which can be prepared by a simple procedure, a method for producing the transformant, and a method for detecting the presence of a reduced phosphorous compound with use of the transformant, a transformant in accordance with an embodiment of the present invention is a transformant which is defective in functions of a gene encoding a phosphate transporter protein and a gene encoding a phosphate ester transporter protein and into which a gene encoding a hypophosphite transporter protein is introduced.

Transformant, method for producing transformant, and method for detecting presence or absence of reduced phosphorus compound using transformant

The present invention provides a method for purifying an intended substance, a kit for purifying the intended substance, and a silicon oxide-binding tag for use in the method and the kit. In the present invention, a complex of a silicon oxide-binding tag and a substance is caused to specifically bind to silicon oxide in a solution for binding, to which solution no salt is added, and binding between the silicon oxide-binding tag and the silicon oxide is broken with use of arginine.

Purification method, purification kit, and silicon oxide-binding tag for use therein

A simple and inexpensive method for selectively culturing a microorganism which method makes it possible to selectively culture a microorganism of interest even without using a sterilization operation or an antibiotic substance is provided. The method according to the present invention selectively culturing a microorganism includes the step of culturing, in a culture medium containing phosphorous acid as a sole phosphorous source, a recombinant microorganism into which a phosphite dehydrogenase gene has been introduced.

Method for selectively culturing microorganism using phosphite dehydrogenase gene as marker

In order to provide a method for more efficiently, easily, and accurately detecting asbestos without changing the asbestos detection criterion of the phase-contrast microscope/electron microscope method as compared with the phase-contrast microscope/electron microscope method, a phase-contrast microscope and a fluorescence microscope are used in combination to detect asbestos contained in a test sample after the test sample is made contact with an asbestos-binding protein having a fluorescent label.

Method for detecting asbestos

In order to provide: a phosphite dehydrogenase protein having both improved solubility and improved heat stability; a method for producing a gene encoding the phosphite dehydrogenase protein; a method for producing the phosphite dehydrogenase protein; and use of the phosphite dehydrogenase protein, (i) a phosphite dehydrogenase protein having a specific amino acid sequence and (ii) a gene encoding the phosphite dehydrogenase protein are used.

Method for producing phosphite dehydrogenase protein and use thereof

The present invention provides a method comprising allowing a reaction of a sample, a reagent containing Factor C, which can be activated by binding with endotoxin, and a synthetic luminescent substrate comprising a luminescent substrate bound to a peptide, for release of the luminescent substrate from the synthetic luminescent substrate, allowing a luminescent enzyme to act on the luminescent substrate released in the luminescent substrate release step, for measurement of the luminescence intensity, and quantifying the level of endotoxin in the sample based on a measured value obtained in the luminescence measuring step, the method enabling endotoxin to be simply and quickly measured at a level that cannot be detected in conventional methods for endotoxin measurement, without use of any dedicated measuring device.

Kit for measurement of endotoxin level by bioluminescent assay

Luminescent method for measuring endotoxin

The present invention provides a prompt and easy asbestos detection method and a method for screening a candidate for an agent aiming at preventing or treating a disease for which asbestos is a causative or worsening factor. It is possible to quickly and easily detect asbestos in a sample by finding a protein capable of binding specifically to asbestos, allowing the protein or a fusion protein of the protein and a reporter protein to bind to asbestos in the sample, and then detecting the protein or the fusion protein having been bound to asbestos. A substance inhibiting the binding of actin to asbestos, which has been found out as a protein capable of binding specifically to asbestos, is a candidate for an agent aiming at preventing or treating a disease for which asbestos is a causative or worsening factor.

Asbestos detection method, asbestos detection agent, asbestos detection kit, method for screening candidate for agent aiming at preventing or treating disease for which asbestos is causative or worsening factor

The present invention provides a method for purifying a protein, includes the step of: contacting a fusion protein of a first protein and a second protein with a bivalent cation-containing solution, the fusion protein being adsorbed to a silicon oxide-containing substance, the first protein being capable of binding to the silicon oxide-containing substance in a solution containing 0.1M sodium chloride. With this arrangement, it is possible to easily produce large quantity of proteins which are high in purity without sacrificing activity of the proteins.

Method for purifying protein

The ATP amplification method is a method for amplifying and detecting a very trace amount of exogenous ATP by allowing a fusion protein (PPK-ADK) of a polyphosphate kinase and an adenylate kinase, the fusion protein not containing ADP, to act on a mixture of ATP, AMP, and a polyphosphate compound. Also provided is an ultrasensitive ATP amplification method by which ATP at a single cell level can be amplified and detected, and an ultrasensitive microbial assay based on this ATP amplification method.

Method of amplifying ATP and use thereof

Growing community of inventors

Hiroshima, Japan

Akio Kuroda