Hamamatsu-photonics K.k.

Akinori Oda

Hiroshi Satozono

Yohei Takata

Tomomi Shinke

Provided is a method of quantifying a target substance present in a solvent comprises acquiring a first fluorescence decay curve and a second fluorescence decay curve to acquire some factors from the curves.

Method for removing background of fluorescence lifetime measurement and method for quantifying target substance

The present invention relates to a method for detecting an amyloid &#x3b2; oligomer which includes a test sample and thioflavin T are brought into contact with each other, fluorescence of the thioflavin T is measured to obtain time-resolved fluorescence spectra, the time-resolved fluorescence spectrum of time period t1 to t2 and the time-resolved fluorescence spectrum of time period t3 to t4 are respectively normalized to obtain normalized spectra (t1&#x3c;t2&#x2264;t3&#x3c;t4), and determination is made to confirm the presence or absence of an amyloid &#x3b2; oligomer in the test sample on the basis of the two normalized spectra, and shifting of the normalized spectrum of time period t3 to t4 towards the low wavelength side in comparison to the normalized spectrum of time period t1 to t2 indicates the presence of an amyloid &#x3b2; oligomer in the test sample.

Amyloid &#x3b2; oligomer detection method, amyloid &#x3b2; oligomer detection device, and amyloid &#x3b2; oligomer detection program

The present invention relates to a method for purifying pure thioflavin T in having a step of preparing a thioflavin T solution in which crude thioflavin T is dissolved in a polar solvent, a step of bringing the thioflavin T solution into contact with a non-polar polymeric porous body, and a step of separating the thioflavin T solution after the contact from the non-polar polymeric porous body.

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Hamamatsu, Japan

Akinori Oda