Ngk Insulators, Inc.

Japan Science and Technology Agency

Other

Isao Saito

Akimitsu Okamoto

Yasuko Yoshida

Kazuo Tanaka

Kousuke Niwa

Yoshio Saito

A method of easily releasing a useful substance bonded to oligonucleotide without impairing a target nucleic acid; and a novel base therefore. A nucleoside of nucleotide (oligonucleotide containing thereof) represented by the formula (I) (wherein each of X and Y independently represents &#x2014;O&#x2014;, &#x2014;NH&#x2014;, &#x2014;N(alkyl)- or &#x2014;S&#x2014;; R represents a functional unit, a reporter unit or a biofunctional molecule; each of Rand Rindependently represents a hydrogen atom, a phosphate bond group, a phosphoramidite group or a nucleotide; and n is a numeral of 1 to 10). There is further provided a method of releasing the R group moiety at base portion by the use of the oligonucleotide comprising the nucleotide.

Nucleoside releasing functional unit through oxidation and process for producing oligonucleotide containing thereof

A novel nucleotide derivative, in case of existing as a member of a single-stranded sequence, undergoing a change in the fluorescent signal intendity depending on the corresponding base type in the partner strand with which the single-stranded sequence is hybridized, and which is a thymin/uracil derivative (1) emitting light most intensely when a confronting base in the partner strand with which the single-stranded nucleotide sequence is hybridized is adenine; a cytosine derivative (2) emitting light most intensely when the confronting base is guanine; an adenine derivative (3) emitting light most intensely when the confronting base is cytosine; a guanine derivative (4) emitting light most intensely when the confronting base is cytosine or thymine/uracil; and an adrnine derivative (5) emitting light most intensely when the confronting base is thymine/uracil/.

Nucleotide derivative and DNA microarray

A novel nucleotide derivative, in case of existing as a member of a single-stranded sequence, undergoing a change in the fluorescent signal intensity depending on the corresponding base type in the partner strand with which the single-stranded sequence is hybridized, and which is (1) a thymine/uracil derivative emitting light most intensely when a confronting base in the partner strand with which the single-stranded nucleotide sequence is hybridized is adenine; (2) a cytosine derivative emitting light most intensely when the confronting base is guanine; (3) an adenine derivative emitting light most intensely when the confronting base is cytosine; and, (4) a guanine derivative emitting light most intensely when the confronting base is cytosine or thymine/uracil.

A nucleic acid base for hole transportation in DNAs which does not cause oxidative decomposition; and an artificial DNA molecule which can realize effective hole transportation in DNAs while maintaining the double spiral structure of the DNAs. Provided are: a nucleic acid which contains a benzodeazaadenine derivative base represented by the general formula (I): (wherein R, R, R, R, R, and Reach independently represents hydrogen, amino, mono (lower alkyl) amino, di (lower alkyl) amino, hydroxy, lower alkoxy, halogeno, cyano, mercapto, lower alkylthio, or aryl; and Rand Reach independently represents hydrogen or a group bonded to phosphoric acid);

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Kyoto, Japan

Akimitsu Okamoto